Articles by Jie Yan in JoVE
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases Dongshan Yang1, Jifeng Zhang1, Jie Xu1, Tianqing Zhu1, Yanbo Fan1, Jianglin Fan2, Y. Eugene Chen1 1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Department of Molecular Pathology, University of Yamanashi Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers Huijuan You*1, Shimin Le*2,3, Hu Chen2,4, Linyan Qin1, Jie Yan2,4,5 1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 2Mechanobiology Institute, National University of Singapore, 3Department of Physics, National University of Singapore, 4Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, Xiamen University, 5Center for Bioimaging Sciences, National University of Singapore A single-molecule magnetic tweezers platform to manipulate G-quadruplexes is reported, which allows for the study of G4 stability and regulation by various proteins.