In JoVE (5)
- Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model
- Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
- Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
- Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
- Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
Other Publications (0)
Articles by Jing Wang in JoVE
Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model Jing Feng1, Yvonne Fitz1, Yan Li1, Melinda Fernandez1, Irene Cortes Puch1, Dong Wang1, Stephanie Pazniokas2, Brandon Bucher3, Xizhong Cui1, Steven B. Solomon1 1Critical Care Medicine Department, Clinical Center, National Institutes of Health, 2Harvard Apparatus, 3ADInstruments Vascular accesses to measure hemodynamics, provide fluids and perform blood sampling are important to any small animal model study. We present a technique for implanting catheters into the carotid artery and the common jugular vein in an anesthetized rat for connecting to a system to perform monitoring, infusions and sampling.
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format Muhammad Khairul Ramlee1, Jing Wang1, Alice M. S. Cheung1, Shang Li1 1Cancer & Stem Cell Biology Programme, Duke-NUS Medical School The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel electrophoresis, allows for high-throughput genotyping of nuclease-mediated knockout clones. It circumvents limitations faced by other genotyping techniques and is more cost effective than sequencing methods.
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method Thomas R. Groves1,2,3, Jing Wang1,2, Marjan Boerma1,2, Antiño R. Allen1,2,3 1Division of Radiation Health, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences Here we present a Golgi-Cox protocol in extensive detail. This reliable tissue stain method allows for a high-quality assessment of the cytoarchitecture in the hippocampus, and throughout the entire brain, with minimal troubleshooting.
Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications Xiangzhao Ai1, Linna Lyu1, Jing Mu1, Ming Hu1, Zhimin Wang1, Bengang Xing1,2 1Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, 2Institute of Materials Research & Engineering, Agency for Science, Technology and Research (A*STAR) A protocol is presented for the synthesis of core-shell lanthanide-doped upconversion nanocrystals (UCNs) and their cellular applications for channel protein regulation upon near-infrared (NIR) light illumination.
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy Jing Guo1, Sifan You1, Zhichang Wang1, Jinbo Peng1, Runze Ma1, Ying Jiang1,2 1International Center for Quantum Materials, School of Physics, Peking University, 2Collaborative Innovation Center of Quantum Matter Here, we present a protocol to investigate the structure and dynamics of interfacial water at the atomic scale, in terms of submolecular resolution imaging, molecular manipulation, and single-bond vibrational spectroscopy.