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Articles by Jo A. Crum in JoVE
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue Thomas Lampl1, Jo A. Crum1, Taylor A. Davis1, Carol Milligan2,3,4, Victoria Del Gaizo Moore1 1Chemistry Department, Elon University, 2Department of Neurobiology and Anatomy, Wake Forest School of Medicine, 3Neuroscience Graduate Program, Wake Forest School of Medicine, 4ALS Center Translational Science Unit, Wake Forest School of Medicine Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.