Johanna F Dekkers

Research

Prinsess Maxima Centre for pediatric oncology

Johanna F Dekkers
Postdoc

Florijn Dekkers works with the human organoid or ‘mini-organ’ technology to faithfully recreate healthy organs or tumors in a dish. She uses these cultures to study the effectiveness of drug treatment in a patient-specific manner. It facilitates the development of therapy that is tailored to the individual. This is of great importance for children with cancer, because they can suffer from serious treatment side effects which often establish later in life.

She also combines organoids with advanced fluorescent microscopy. These methods include large-scale single cell-resolution 3D imaging on fixed tissue and 3D live-cell imaging in vitro and in vivo. She develops innovative approaches to visualize cancer biology in a unique way to thereby better understand the progression of cancer and the mechanism-of-action of anticancer therapy, such as immunotherapy with engineered T cells. She currently mainly applies her studies on breast cancer, but will translate her methods to study pediatric cancer.

Publications

הדמיה תלת-ממדית ברזולוציה חד-תאית של אורגנואידים שלמים

1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

JoVE 60709

 Biology

Forskolin-induced נפיחות מעיים Organoids: An

1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Department of Stem Cells and Cancer, Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht

 Medicine