In JoVE (1)
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Articles by John Edward Connolly in JoVE
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes Wen Jing Sim1, Frano Malinarich1, Anna-Marie Fairhurst2, John Edward Connolly1,3 1Translational Immunology, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies, Baylor University Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.
Other articles by John Edward Connolly on PubMed
Metabolism Is Central to Tolerogenic Dendritic Cell Function Mediators of Inflammation. 2016 | Pubmed ID: 26980944 Immunological tolerance is a fundamental tenant of immune homeostasis and overall health. Self-tolerance is a critical component of the immune system that allows for the recognition of self, resulting in hyporeactivity instead of immunogenicity. Dendritic cells are central to the establishment of dominant immune tolerance through the secretion of immunosuppressive cytokines and regulatory polarization of T cells. Cellular metabolism holds the key to determining DC immunogenic or tolerogenic cell fate. Recent studies have demonstrated that dendritic cell maturation leads to a shift toward a glycolytic metabolic state and preferred use of glucose as a carbon source. In contrast, tolerogenic dendritic cells favor oxidative phosphorylation and fatty acid oxidation. This dichotomous metabolic reprogramming of dendritic cells drives differential cellular function and plays a role in pathologies, such as autoimmune disease. Pharmacological alterations in metabolism have promising therapeutic potential.