In JoVE (1)
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Articles by Jose Ruiz Ramirez in JoVE
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination Suchismita Das1, Lindsey Garver1, Jose Ruiz Ramirez1, Zhiyong Xi1, George Dimopoulos1 1Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.