Articles by Josephine Iaria in JoVE
Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System Hao Chen*1, Thomas M.B. Ware*1, Josephine Iaria1, Hong-Jian Zhu1 1Department of Surgery (RMH), University of Melbourne Here, we present a protocol for live cell imaging of TGF-β/Smad3 signaling activity using an adenovirus reporter system. This system tracks transcriptional activity in real-time and can be applied to both single cells in vitro and in live animalmodels.
Other articles by Josephine Iaria on PubMed
New Reagents for Improved in Vitro and in Vivo Examination of TGF-β Signalling Growth Factors (Chur, Switzerland). Oct, 2011 | Pubmed ID: 21913800 Transforming growth factor-β (TGF-β) signalling controls many aspects of cell behaviour and is implicated as a key regulator in tumour formation and progression. However, evaluating levels of active TGF-β in culture medium or patient plasma and gaining definitive information regarding the activity of downstream substrates such as Sma- and Mad-related protein 3 (Smad3) in vivo with accuracy and sensitivity has been problematic. Therefore, to overcome these technical issues we have created a NIH3T3 cell line with stable pCAGA(12)-luc expression that can now be utilised to detect TGF-β activity with high sensitivity. In addition, we have created an adenoviral Smad3 luciferase reporter construct pAd.CAGA(12)-luc to successfully infect cells for in vitro assays, or prior to injection into mice and used to measure transcriptional activity in vivo. Thus, the NIH3T3-pCAGA(12)-luc cell line and the pAd.CAGA(12)-luc adenovirus will be extremely useful tools to measure TGF-β signalling activity with far greater efficiency and reliability compared to original and currently used reagents.
Single Live Cell TGF-β Signalling Imaging: Breast Cancer Cell Motility and Migration is Driven by Sub-populations of Cells with Dynamic TGF-β-Smad3 Activity Molecular Cancer. Feb, 2015 | Pubmed ID: 25744371 Metastasis is a process where only a small subset of cells is capable of successfully migrating to and propagating at secondary sites. TGF-β signalling is widely known for its role in cancer metastasis and is associated with cell migration in whole cell populations.
Ras Enhances TGF-β Signaling by Decreasing Cellular Protein Levels of Its Type II Receptor Negative Regulator SPSB1 Cell Communication and Signaling : CCS. Mar, 2018 | Pubmed ID: 29534718 Transformation by oncogene Ras overcomes TGF-β mediated growth inhibition in epithelial cells. However, it cooperates with each other to mediate epithelial to mesenchymal transition (EMT). The mechanism of how these two pathways interact with each other is controversial.