Articles by Joshua Bush in JoVE
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects Gary Balian1, Gina Beck1, Vedavathi Madhu1, Robert Sikes2, Quanjun Cui3, Haixiang Liang1, Joshua Bush1 1Orthopaedics Research, University of Virginia, 2Biological Sciences, University of Delaware, 3Orthopaedic Surgery, University of Virginia A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these peptides on mesenchymal cell differentiation and to determine their effect on bone regeneration.
Other articles by Joshua Bush on PubMed
Improved Bio-implant Using Ultrafast Laser Induced Self-assembled Nanotexture in Titanium Journal of Biomedical Materials Research. Part B, Applied Biomaterials. May, 2011 | Pubmed ID: 21394901 The most successful metal implant materials currently have relatively smooth surfaces on the micron size scale, with most failures occurring after only 10 years. To move beyond this limiting time scale, texturing methods have been developed to modify the metal surface to enhance integration of the implant directly with surrounding bone. A flexible single-step ultrafast-laser texturing process has been developed that results in a surface texture that exhibits micron scale peaks and troughs with superimposed submicron and nano-scale features. The textured titanium samples remain completely hydrophilic with no measurable contact angle even after several weeks in normal atmosphere. An increase in mesenchymal stem cell number is observed over that on an untreated control titanium surface. Extensive formation of cellular bridges by stromal cells between pillars shows the favorable response of differentiated cells to the surface and the promotion of their attachment. Expression of the alkaline phosphatase and osteocalcin genes in human bone marrow cells were seen to increase on the textured surface. The development of this single-step method for creating micron, submicron, and nano-scale surface texture directly on metals makes a significant contribution to the goal of improving the integration and life span of joint replacement implants.