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Articles by Julia Dawitz in JoVE
Functional Calcium Imaging in Developing Cortical Networks Julia Dawitz1, Tim Kroon1, J.J. Johannes Hjorth1, Rhiannon M. Meredith1 1Department of Integrative Neurophysiology, VU University, Amsterdam Spontaneous activity of developing neuronal networks can be measured using AM-ester forms of calcium-sensitive indicator dyes. Changes in intracellular calcium, indicating neuronal activation, are detected as transient changes in indicator fluorescence with one- or two-photon imaging. This protocol can be adapted for a range of developmentally-dependent neuronal networks in vitro.