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Articles by Julien Besnard in JoVE
Glutamine Flux Imaging Using Genetically Encoded Sensors Julien Besnard1, Sakiko Okumoto1 1Virginia Tech This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.