Other Publications (1)
Articles by Jung Min Lee in JoVE
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology Thomas G. Schuhmann Jr.1, Tao Zhou2, Guosong Hong2, Jung Min Lee2,3, Tian-Ming Fu2, Hong-Gyu Park3, Charles M. Lieber1,2 1John A. Paulson School of Engineering and Applied Sciences, Harvard University, 2Department of Chemistry and Chemical Biology, Harvard University, 3Department of Physics, Korea University Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
Other articles by Jung Min Lee on PubMed
A Method for Single-neuron Chronic Recording from the Retina in Awake Mice Science (New York, N.Y.). | Pubmed ID: 29954976 The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.