Other Publications (1)
Articles by Junhwan Kwon in JoVE
Spectral Reflectometric Microscopy on Myelinated Axons In Situ Junhwan Kwon1,2, Myunghwan Choi1,2 1Department of Biomedical Engineering, Sungkyunkwan University, 2Center for Neuroscience Imaging Research, Institute for Basic Science (IBS) Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.
Other articles by Junhwan Kwon on PubMed
Label-free Nanoscale Optical Metrology on Myelinated Axons in Vivo Nature Communications. Nov, 2017 | Pubmed ID: 29184114 In the mammalian nervous system, myelin provides electrical insulation for the neural circuit by forming a highly organized, multilayered thin film around the axon fibers. Here, we investigate the spectral reflectance from this subcellular nanostructure and devise a new label-free technique based on a spectroscopic analysis of reflected light, enabling nanoscale imaging of myelinated axons in their natural living state. Using this technique, we demonstrate three-dimensional mapping of the axon diameter and sensing of dynamic changes in the substructure of myelin at nanoscale. We further reveal the prevalence of axon bulging in the brain cortex in vivo after mild compressive trauma. Our novel tool opens new avenues of investigation by creating unprecedented access to the nanostructural dynamics of live myelinated axons in health and disease.