In JoVE (1)
Other Publications (1)
Articles by Kannan Menon in JoVE
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition Jeremy J. Flint1,2, Kannan Menon2,3, Brian Hansen4, John Forder2,3,5, Stephen J. Blackband1,2,6 1Department of Neuroscience, University of Florida, 2McKnight Brain Institute, University of Florida, 3Department of Biomedical Engineering, University of Florida, 4Center for Functionally Integrative Neuroscience, Aarhus University, 5Department of Radiology, University of Florida, 6National High Magnetic Field Laboratory, Florida State University The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy data.
Other articles by Kannan Menon on PubMed
A Microperfusion and In-Bore Oxygenator System Designed for Magnetic Resonance Microscopy Studies on Living Tissue Explants Scientific Reports. Dec, 2015 | Pubmed ID: 26666980 Spectrometers now offer the field strengths necessary to visualize mammalian cells but were not designed to accommodate imaging of live tissues. As such, spectrometers pose significant challenges--the most evident of which are spatial limitations--to conducting experiments in living tissue. This limitation becomes problematic upon trying to employ commercial perfusion equipment which is bulky and--being designed almost exclusively for light microscopy or electrophysiology studies--seldom includes MR-compatibility as a design criterion. To overcome problems exclusive to ultra-high magnetic field environments with limited spatial access, we have designed microperfusion and in-bore oxygenation systems capable of interfacing with Bruker's series of micro surface-coils. These devices are designed for supporting cellular resolution imaging in MR studies of excised, living tissue. The combined system allows for precise control of both dissolved gas and pH levels in the perfusate thus demonstrating applicability for a wide range of tissue types. Its compactness, linear architecture, and MR-compatible material content are key design features intended to provide a versatile hardware interface compatible with any NMR spectrometer. Such attributes will ensure the microperfusion rig's continued utility as it may be used with a multitude of contemporary NMR systems in addition to those which are currently in development.