Articles by Kanwardeep S. Kaleka in JoVE
Pull-down da Calmodulina Proteínas de Ligação Kanwardeep S. Kaleka1, Amber N. Petersen1, Matthew A. Florence1, Nashaat Z. Gerges1 1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin Calmodulina (CaM) ensaio de pull-down é uma maneira eficaz para investigar a interação de CaM com várias proteínas. Este método usa CaM-sepharose contas para análise eficiente e específica do CaM proteínas de ligação. Isto proporciona uma importante ferramenta para explorar sinalização CaM em função celular.
Other articles by Kanwardeep S. Kaleka on PubMed
Neurogranin Phosphorylation Fine-tunes Long-term Potentiation The European Journal of Neuroscience. Jan, 2011 | Pubmed ID: 21198977 Learning-related potentiation of synaptic strength at Cornu ammonis subfield 1 (CA1) hippocampal excitatory synapses is dependent on neuronal activity and the activation of glutamate receptors. However, molecular mechanisms that regulate and fine-tune the expression of long-term potentiation (LTP) are not well understood. Recently it has been indicated that neurogranin (Ng), a neuron-specific, postsynaptic protein that is phosphorylated by protein kinase C, potentiates synaptic transmission in an LTP-like manner. Here, we report that a Ng mutant that is unable to be phosphorylated cannot potentiate synaptic transmission in rat CA1 hippocampal neurons and results in a submaximal expression of LTP. Our results provide the first evidence that the phosphorylation of Ng can regulate LTP expression.