Karen M. Polizzi Department of Chemical Engineering Imperial College London Biography Publications Institution JoVE Articles Karen M. Polizzi has not added a biography. If you are Karen M. Polizzi and would like to personalize this page please email our Author Liaison for assistance. Publications Cell-Free Protein Synthesis Using Pichia Pastoris Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34985738 Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34611868 Production and Purification of an Active CRM197 in Pichia Pastoris and Its Immunological Characterization Using a Vi-typhoid Antigen Vaccine Vaccine. 12, 2021 | Pubmed ID: 34774362 Model Identifies Genetic Predisposition of Alzheimer's Disease As Key Decider in Cell Susceptibility to Stress International Journal of Molecular Sciences. Nov, 2021 | Pubmed ID: 34769426 Cell-free Protein Synthesis Using Chinese Hamster Ovary Cells Methods in Enzymology. 2021 | Pubmed ID: 34752298 Expression of Proteins in Pichia Pastoris Methods in Enzymology. 2021 | Pubmed ID: 34742398 High-yield 'one-pot' Biosynthesis of Raspberry Ketone, a High-value Fine Chemical Synthetic Biology (Oxford, England). 2021 | Pubmed ID: 34712844 GenoChemetic Strategy for Derivatization of the Violacein Natural Product Scaffold ACS Chemical Biology. 11, 2021 | Pubmed ID: 34648268 Hitting the Sweet Spot with Capillary Electrophoresis: Advances in N-glycomics and Glycoproteomics Current Opinion in Biotechnology. 10, 2021 | Pubmed ID: 34438131 Correction To: Refactoring of a Synthetic Raspberry Ketone Pathway with EcoFlex Microbial Cell Factories. Aug, 2021 | Pubmed ID: 34407814 An Experimental Approach Probing the Conformational Transitions and Energy Landscape of Antibodies: a Glimmer of Hope for Reviving Lost Therapeutic Candidates Using Ionic Liquid Chemical Science. Jul, 2021 | Pubmed ID: 34349928 Refactoring of a Synthetic Raspberry Ketone Pathway with EcoFlex Microbial Cell Factories. Jun, 2021 | Pubmed ID: 34112158 Knockout of RSN1, TVP18 or CSC1-2 Causes Perturbation of Golgi Cisternae in Pichia Pastoris Traffic (Copenhagen, Denmark). 03, 2021 | Pubmed ID: 33263222 Microbial Interventions Are an Easier Alternative to Engineer Higher Organisms Microbial Biotechnology. 01, 2021 | Pubmed ID: 33070407 Exploring Conformational Preferences of Proteins: Ionic Liquid Effects on the Energy Landscape of Avidin Chemical Science. Oct, 2020 | Pubmed ID: 34163590 Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform Frontiers in Bioengineering and Biotechnology. 2020 | Pubmed ID: 33604330 Methods for Expression of Recombinant Proteins Using a Pichia Pastoris Cell-Free System Current Protocols in Protein Science. 12, 2020 | Pubmed ID: 33108045 Improving the Reaction Mix of a Cell-free System Using a Design of Experiments Approach to Minimise Experimental Effort Synthetic and Systems Biotechnology. Sep, 2020 | Pubmed ID: 32637667 Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Cell-Free System Frontiers in Bioengineering and Biotechnology. 2020 | Pubmed ID: 32117947 BeQuIK (Biosensor Engineered Quorum Induced Killing): Designer Bacteria for Destroying Recalcitrant Biofilms Microbial Biotechnology. 03, 2020 | Pubmed ID: 31328393 A Label-free Optical Whole-cell Escherichia Coli Biosensor for the Detection of Pyrethroid Insecticide Exposure Scientific Reports. 08, 2019 | Pubmed ID: 31462650 Low-cost and User-friendly Biosensor to Test the Integrity of MRNA Molecules Suitable for Field Applications Biosensors & Bioelectronics. Jul, 2019 | Pubmed ID: 31100599 Whole-Cell Biosensor with Tunable Limit of Detection Enables Low-Cost Agglutination Assays for Medical Diagnostic Applications ACS Sensors. 02, 2019 | Pubmed ID: 30623662 Biosensor-assisted Engineering of a High-yield Pichia Pastoris Cell-free Protein Synthesis Platform Biotechnology and Bioengineering. 03, 2019 | Pubmed ID: 30552674 Tools for Engineering Coordinated System Behaviour in Synthetic Microbial Consortia Nature Communications. 07, 2018 | Pubmed ID: 29992956 Investigating the Consequences of Asymmetric Endoplasmic Reticulum Inheritance in Under Stress Using a Combination of Single Cell Measurements and Mathematical Modelling Synthetic and Systems Biotechnology. Mar, 2018 | Pubmed ID: 29911200 Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation Cell Systems. Apr, 2018 | Pubmed ID: 29680377 Rapid Acquisition and Model-based Analysis of Cell-free Transcription-translation Reactions from Nonmodel Bacteria Proceedings of the National Academy of Sciences of the United States of America. 05, 2018 | Pubmed ID: 29666238 A Systematic Analysis of the Expression of the Anti-HIV VRC01 Antibody in Pichia Pastoris Through Signal Peptide Optimization Protein Expression and Purification. 09, 2018 | Pubmed ID: 29601964 Exploring Cellular Behavior Under Transient Gene Expression and Its Impact on MAb Productivity and Fc-glycosylation Biotechnology and Bioengineering. 02, 2018 | Pubmed ID: 28921534 A Streamlined Cloning Workflow Minimising the Time-to-strain Pipeline for Pichia Pastoris Scientific Reports. Nov, 2017 | Pubmed ID: 29150665 Sense and Sensitivity in Bioprocessing-detecting Cellular Metabolites with Biosensors Current Opinion in Chemical Biology. Oct, 2017 | Pubmed ID: 28609710 Expressing Anti-HIV VRC01 Antibody Using the Murine IgG1 Secretion Signal in Pichia Pastoris AMB Express. Dec, 2017 | Pubmed ID: 28342171 Whole-cell Escherichia Coli Lactate Biosensor for Monitoring Mammalian Cell Cultures During Biopharmaceutical Production Biotechnology and Bioengineering. 06, 2017 | Pubmed ID: 28112405 Model-based Investigation of Intracellular Processes Determining Antibody Fc-glycosylation Under Mild Hypothermia Biotechnology and Bioengineering. 07, 2017 | Pubmed ID: 27869292 A Theoretical Estimate for Nucleotide Sugar Demand Towards Chinese Hamster Ovary Cellular Glycosylation Scientific Reports. 06, 2016 | Pubmed ID: 27345611 Liquid PTVA: a Faster and Cheaper Alternative for Generating Multi-copy Clones in Pichia Pastoris Microbial Cell Factories. Feb, 2016 | Pubmed ID: 26849882 How Does Mild Hypothermia Affect Monoclonal Antibody Glycosylation? Biotechnology and Bioengineering. Jun, 2015 | Pubmed ID: 25545631 Genetically-encoded Biosensors for Monitoring Cellular Stress in Bioprocessing Current Opinion in Biotechnology. Feb, 2015 | Pubmed ID: 25151059 Co-culture Systems and Technologies: Taking Synthetic Biology to the Next Level Journal of the Royal Society, Interface. Jul, 2014 | Pubmed ID: 24829281 Towards Controlling the Glycoform: a Model Framework Linking Extracellular Metabolites to Antibody Glycosylation International Journal of Molecular Sciences. Mar, 2014 | Pubmed ID: 24637934 Can Too Many Copies Spoil the Broth? Microbial Cell Factories. 2013 | Pubmed ID: 24354594 Opportunities for Bioprocess Monitoring Using FRET Biosensors Biochemical Society Transactions. Oct, 2013 | Pubmed ID: 24059500 Comparative Analysis of Amino Acid Metabolism and Transport in CHO Variants with Different Levels of Productivity Journal of Biotechnology. Dec, 2013 | Pubmed ID: 24056080 An Optimized Method for Extraction and Quantification of Nucleotides and Nucleotide Sugars from Mammalian Cells Analytical Biochemistry. Sep, 2013 | Pubmed ID: 24036437 What is Synthetic Biology? Methods in Molecular Biology (Clifton, N.J.). 2013 | Pubmed ID: 23996434 In Situ Monitoring of Intracellular Glucose and Glutamine in CHO Cell Culture PloS One. 2012 | Pubmed ID: 22509313 Binding of Human BiP to the ER Stress Transducers IRE1 and PERK Requires ATP Biochemical and Biophysical Research Communications. Apr, 2012 | Pubmed ID: 22446326 Protein Construct Optimization: Data Sharing Strategy Protein & Cell. May, 2012 | Pubmed ID: 22410785 The Role of ER Stress-induced Apoptosis in Neurodegeneration Current Alzheimer Research. Mar, 2012 | Pubmed ID: 22299619 Genome-based Kinetic Modeling of Cytosolic Glucose Metabolism in Industrially Relevant Cell Lines: Saccharomyces Cerevisiae and Chinese Hamster Ovary Cells Bioprocess and Biosystems Engineering. Aug, 2012 | Pubmed ID: 22286123 Metabolic Network Reconstruction: Advances in in Silico Interpretation of Analytical Information Current Opinion in Biotechnology. Feb, 2012 | Pubmed ID: 22119273 Revealing Biases Inherent in Recombination Protocols BMC Biotechnology. 2007 | Pubmed ID: 18001472 Stability of Biocatalysts Current Opinion in Chemical Biology. Apr, 2007 | Pubmed ID: 17307381 Better Library Design: Data-driven Protein Engineering Biotechnology Journal. Feb, 2007 | Pubmed ID: 17183506 High-throughput Screening for Enhanced Protein Stability Current Opinion in Biotechnology. Dec, 2006 | Pubmed ID: 17049838 Structure-guided Consensus Approach to Create a More Thermostable Penicillin G Acylase Biotechnology Journal. May, 2006 | Pubmed ID: 16892288 Pooling for Improved Screening of Combinatorial Libraries for Directed Evolution Biotechnology Progress. Jul-Aug, 2006 | Pubmed ID: 16889370 Simulation Modeling of Pooling for Combinatorial Protein Engineering Journal of Biomolecular Screening. Dec, 2005 | Pubmed ID: 16234344 Glicoengenia de anticorpos rápidos em células de ovário de hamster chinês Masue Marbiah*1,2, Pavlos Kotidis*1,3, Roberto Donini1,4, Itzcóatl A. Gómez5, Ioscani Jimenez del Val5, Stuart M. Haslam4, Karen M. Polizzi1,2, Cleo Kontoravdi1 1Department of Chemical Engineering, Imperial College London, 2Imperial College Centre for Synthetic Biology, Imperial College London, 3BioPharm Process Research, GlaxoSmithKline Research and Development, 4Department of Life Science, Imperial College London, 5School of Chemical & Bioprocess Engineering, University College Dublin JoVE 63872 Bioengineering FIBS habilitado Noninvasive Metabolic Profiling Alireza Behjousiar1,2, Antony Constantinou2, Karen M. Polizzi2, Cleo Kontoravdi1 1Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology, Imperial College London, 2Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences, Imperial College London JoVE 51200 Bioengineering
Glicoengenia de anticorpos rápidos em células de ovário de hamster chinês Masue Marbiah*1,2, Pavlos Kotidis*1,3, Roberto Donini1,4, Itzcóatl A. Gómez5, Ioscani Jimenez del Val5, Stuart M. Haslam4, Karen M. Polizzi1,2, Cleo Kontoravdi1 1Department of Chemical Engineering, Imperial College London, 2Imperial College Centre for Synthetic Biology, Imperial College London, 3BioPharm Process Research, GlaxoSmithKline Research and Development, 4Department of Life Science, Imperial College London, 5School of Chemical & Bioprocess Engineering, University College Dublin JoVE 63872 Bioengineering
FIBS habilitado Noninvasive Metabolic Profiling Alireza Behjousiar1,2, Antony Constantinou2, Karen M. Polizzi2, Cleo Kontoravdi1 1Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology, Imperial College London, 2Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences, Imperial College London JoVE 51200 Bioengineering
