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Articles by Katie L. DeCicco-Skinner in JoVE
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis Katie L. DeCicco-Skinner1, Gervaise H. Henry1, Christophe Cataisson2, Tracy Tabib1, J. Curtis Gwilliam1, Nicholas J. Watson1, Erica M. Bullwinkle1, Lauren Falkenburg1, Rebecca C. O'Neill1, Adam Morin1, Jonathan S. Wiest2 1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.