Articles by Katrina P. Nguyen in JoVE
Feeding Experimentation Device (FED): Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents Katrina P. Nguyen1, Mohamed A. Ali1, Timothy J. O'Neal1, Ilona Szczot1, Julia A. Licholai1,2, Alexxai V. Kravitz1,3 1National Insttitute of Diabetes and Digestive and Kidney Diseases, 2National Center for Complementary and Integrative Health, 3National Institute on Drug Abuse, National Institutes of Health Feeding Experimentation Device (FED) is an open-source device for measuring food intake in mice. FED can also synchronize food intake measurements with other techniques via a real-time digital output. Here, we provide a step-by-step tutorial for the construction, validation, and usage of FED.
Other articles by Katrina P. Nguyen on PubMed
ROBucket: A Low Cost Operant Chamber Based on the Arduino Microcontroller Behavior Research Methods. Jun, 2016 | Pubmed ID: 26019006 The operant conditioning chamber is a cornerstone of animal behavioral research. Operant boxes are used to assess learning and motivational behavior in animals, particularly for food and drug reinforcers. However, commercial operant chambers cost several thousands of dollars. We have constructed the Rodent Operant Bucket (ROBucket), an inexpensive and easily assembled open-source operant chamber based on the Arduino microcontroller platform, which can be used to train mice to respond for sucrose solution or other liquid reinforcers. The apparatus contains two nose pokes, a drinking well, and a solenoid-controlled liquid delivery system. ROBucket can run fixed ratio and progressive ratio training schedules, and can be programmed to run more complicated behavioral paradigms. Additional features such as motion sensing and video tracking can be added to the operant chamber through the array of widely available Arduino-compatible sensors. The design files and programming code are open source and available online for others to use.
Feeding Experimentation Device (FED): A Flexible Open-source Device for Measuring Feeding Behavior Journal of Neuroscience Methods. Jul, 2016 | Pubmed ID: 27060385 Measuring food intake in rodents is a conceptually simple yet labor-intensive and temporally-imprecise task. Most commonly, food is weighed manually, with an interval of hours or days between measurements. Commercial feeding monitors are excellent, but are costly and require specialized caging and equipment.