Other Publications (1)
Articles by Katy M. Monteith in JoVE
Oral Bacterial Infection and Shedding in Drosophila melanogaster Jonathon A. Siva-Jothy1, Arun Prakash1, Radhakrishnan B. Vasanthakrishnan2, Katy M. Monteith1, Pedro F. Vale1,3 1Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, 2IGDR - CNRS UMR 6290, 3Centre for Immunity, Infection and Evolution, University of Edinburgh This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial infection.
Other articles by Katy M. Monteith on PubMed
The Route of Infection Determines Antibacterial Protection in Proceedings. Biological Sciences. | Pubmed ID: 28592678 Bacterial symbionts are widespread among metazoans and provide a range of beneficial functions. -mediated protection against viral infection has been extensively demonstrated in In mosquitoes that are artificially transinfected with (wMel), protection from both viral and bacterial infections has been demonstrated. However, no evidence for -mediated antibacterial protection has been demonstrated in to date. Here, we show that the route of infection is key for -mediated antibacterial protection. carrying showed reduced mortality during enteric-but not systemic-infection with the opportunist pathogen -mediated protection was more pronounced in male flies and is associated with increased early expression of the antimicrobial peptide , and also increased expression of a reactive oxygen species detoxification gene (). These results highlight that the route of infection is important for symbiont-mediated protection from infection, that can protect hosts by eliciting a combination of resistance and disease tolerance mechanisms, and that these effects are sexually dimorphic. We discuss the importance of using ecologically relevant routes of infection to gain a better understanding of symbiont-mediated protection.