Articles by Kelly Shea in JoVE
Dissection של 6.5 עוברים DPC עכבר Kelly Shea1, Niels Geijsen1 1Massachusetts General Hospital, Harvard Stem Cell Institute, Harvard Medical School בידוד של postimplantation בשלב עוברי מאפשר ללמוד דפוסים גן ולנתח התא השושלת תהליכי קבלת ההחלטות במהלך ההתפתחות העוברית, אבל לנתיחה תקין של העובר מוקדם יכול להיות מאתגר. פרוטוקול זה מתאר שיטה לבידוד מוקדם פס פרימיטיבי, בשלב עוברי (~ 6.5 ימים הודעה coitum [DPC]).
Other articles by Kelly Shea on PubMed
Reengineering a Pharmacist Intervention Program American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists. May, 2002 | Pubmed ID: 12040727 At the beginning of a period of rapid growth in clinical pharmacy services in our integrated health system, we realized that there was no mechanism to address how pharmacist interventions were processed, evaluated, and followed up. Interventions were inconsistently documented, and the documentation served no more purpose than to record workload statistics. There was little assessment of the value or quality of interventions, no peer review, and no reporting of aggregate data to improve system-related problems. How are others handling the documentation and assessment of pharmacist interventions?
Sprouty1 Regulates Reversible Quiescence of a Self-renewing Adult Muscle Stem Cell Pool During Regeneration Cell Stem Cell. Feb, 2010 | Pubmed ID: 20144785 Satellite cells are skeletal muscle stem cells capable of self-renewal and differentiation after transplantation, but whether they contribute to endogenous muscle fiber repair has been unclear. The transcription factor Pax7 marks satellite cells and is critical for establishing the adult satellite cell pool. By using a lineage tracing approach, we show that after injury, quiescent adult Pax7(+) cells enter the cell cycle; a subpopulation returns to quiescence to replenish the satellite cell compartment, while others contribute to muscle fiber formation. We demonstrate that Sprouty1 (Spry1), a receptor tyrosine kinase signaling inhibitor, is expressed in quiescent Pax7(+) satellite cells in uninjured muscle, downregulated in proliferating myogenic cells after injury, and reinduced as Pax7(+) cells re-enter quiescence. We show that Spry1 is required for the return to quiescence and homeostasis of the satellite cell pool during repair. Our results therefore define a role for Spry1 in adult muscle stem cell biology and tissue repair.