Articles by Kendra A. Takle in JoVE
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment Destiny L. Baars1, Kendra A. Takle*1,2, Jonathon Heier*1,3, Francisco Pelegri1 1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.
Other articles by Kendra A. Takle on PubMed
Ploidy Manipulation and Induction of Alternate Cleavage Patterns Through Inhibition of Centrosome Duplication in the Early Zebrafish Embryo Developmental Dynamics : an Official Publication of the American Association of Anatomists. Oct, 2015 | Pubmed ID: 26249657 Whole genome duplication is a useful genetic tool because it allows immediate and complete genetic homozygosity in gynogenetic offspring. A whole genome duplication method in zebrafish, Heat Shock, involves a heat pulse in the period 13-15 min postfertilization (mpf) to inhibit cytokinesis of the first mitotic cycle. However, Heat Shock produces a relatively low yield of gynogenotes.