Other Publications (1)
Articles by Kenneth R. Jeffries in JoVE
A Chronic Cardiac Ischemia Model in Swine Using an Ameroid Constrictor Karen J. Keeran*1, Kenneth R. Jeffries1, Arthur D. Zetts1, Joni Taylor1, Shawn Kozlov1, Timothy J. Hunt*1 1Animal Surgery and Resources Core, National Heart, Lung, and Blood Institute, National Institutes of Health The purpose of this protocol is to demonstrate the placement of a delayed constricting device (an ameroid constrictor) around a coronary artery in a swine model. This device creates an ischemic area of the heart that is useful for studying new diagnostic imaging techniques and new methods of treatment.
Other articles by Kenneth R. Jeffries on PubMed
Dendritic Cells Induce Th2-mediated Airway Inflammatory Responses to House Dust Mite Via DNA-dependent Protein Kinase Nature Communications. Feb, 2015 | Pubmed ID: 25692509 DNA-dependent protein kinase (DNA-PK) mediates double-stranded DNA break repair, V(D)J recombination and immunoglobulin class switch recombination, as well as innate immune and pro-inflammatory responses. However, there is limited information regarding the role of DNA-PK in adaptive immunity mediated by dendritic cells (DCs), which are the primary antigen-presenting cells in allergic asthma. Here we show that house dust mite induces DNA-PK phosphorylation, which is a marker of DNA-PK activation, in DCs via the generation of intracellular reactive oxygen species. We also demonstrate that pharmacological inhibition of DNA-PK, as well as the specific deletion of DNA-PK in DCs, attenuates the induction of allergic sensitization and Th2 immunity via a mechanism that involves the impaired presentation of mite antigens. Furthermore, pharmacological inhibition of DNA-PK following antigen priming similarly reduces the manifestations of mite-induced airway disease. Collectively, these findings suggest that DNA-PK may be a potential target for treatment of allergic asthma.