Other Publications (1)
Articles by Kenta Morimoto in JoVE
Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia Kenta Morimoto1,2, Yoichiro Tamori1 1Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science, SOKENDAI, 2Graduate School of Media and Governance, Keio University Mosaic clone analysis in Drosophila imaginal disc epithelia is a powerful model system to study the genetic and cellular mechanisms of tumorigenesis. Here we describe a protocol to induce tumors in Drosophila wing imaginal discs using the GAL4-UAS system, and introduce a diagnosis method to classify the tumor phenotypes.
Other articles by Kenta Morimoto on PubMed
Sirtuin Inhibitor Ex-527 Causes Neural Tube Defects, Ventral Edema Formations, and Gastrointestinal Malformations in Xenopus Laevis Embryos Development, Growth & Differentiation. Aug, 2014 | Pubmed ID: 25131500 Chemical reagent Ex-527 is widely used as a major inhibitor of Sirtuin enzymes, which are a family of highly conserved protein deacetylases and have been linked with caloric restriction and aging by modulating energy metabolism, genomic stability, and stress resistance. However, the extent to which Ex-527 controls early developmental events of vertebrate embryos remains to be understood. Here, we report an examination of Ex-527 effects during Xenopus early development, followed by a confirmation of expressions of xSirt1 and xSirt2 in embryonic stages and enhancement of acetylation by Ex-527. First, we found that reductions in size of neural plate at neurula stages were induced by Ex-527 treatment. Second, tadpoles with short body length and large edematous swellings in the ventral side were frequently observed. Moreover, Ex-527-treated embryos showed severe gastrointestinal malformations in late tadpole stages. Taken together with these results, we conclude that the Sirtuin family start functioning at early embryonic stages and is required for various developmental events.