In JoVE (1)
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Articles by Klara Malinowska in JoVE
Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy Markus A. Jobst*1, Constantin Schoeler*1, Klara Malinowska1, Michael A. Nash1 1Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität Cellulosomes are multienzyme complexes designed for digesting cellulose. AFM-based SMFS was used to study the mechanical properties and folding configuration of cellulosome-associated protein assemblies. We present a complete workflow for protein immobilization, data acquisition, and data analysis to study the interactions of individual receptor-ligand complexes involved in cellulosome assembly.