In JoVE (1)
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Articles by Kristen C. Dias in JoVE
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells Erin L. Sternburg1, Kristen C. Dias1, Fedor V. Karginov1 1Cell Biology and Neuroscience, University of California, Riverside Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.