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Articles by Kumar Perinbam in JoVE
A Rapid Image-based Bacterial Virulence Assay Using Amoeba Kumar Perinbam1, Albert Siryaporn1,2 1Department of Physics and Astronomy, University of California, 2Department of Molecular Biology and Biochemistry, University of California Here, we present a protocol to measure the virulence of planktonic or surface-attached bacteria using D. discoideum (amoeba) as a host. Virulence is measured over a period of 1 h and host killing is quantified using fluorescence microscopy and image analysis. We demonstrate this protocol using the bacterium P. aeruginosa.