Articles by Kyle S. Nickey

In JoVE (1)

Teratom Generation i testis Capsule

Other Publications (1)

PloS One

Articles by Kyle S. Nickey in JoVE

Medicine

Teratom Generation i testis Capsule Suzanne E. Peterson¹, Ha T. Tran¹, Ibon Garitaonandia¹, Sangyoon Han¹, Kyle S. Nickey¹, Trevor Leonardo², Louise C. Laurent³, Jeanne F. Loring¹ ¹Department of Chemical Physiology, Scripps Research Institute, ²Department of Chemical Physiology, Scripps Research Institute, ³Department of Reproductive Medicine, University of California Humane pluripotent stamceller (hPSCs) har potensial til å behandle en myriade av ulike sykdommer. Nytten av disse cellene ligger i det faktum at de kan differensiere i hvilken som helst celletype i kroppen. Her beskriver vi teratom analysen, som brukes til å demonstrere pluripotence av hPSCs.

Other articles by Kyle S. Nickey on PubMed

Normal Human Pluripotent Stem Cell Lines Exhibit Pervasive Mosaic Aneuploidy PloS One. 2011 | Pubmed ID: 21857983 Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC--including induced pluripotent--lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells. This mosaic aneuploidy resembles that observed in progenitor cells of the developing brain and preimplantation embryos, suggesting that it is a normal, rather than pathological, feature of stem cell lines. The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation.

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