Articles by Kyuichi Taguma in JoVE
Criopreservação de embriões de camundongos por Vitrificação Etileno Glicol-Based Keiji Mochida1, Ayumi Hasegawa1, Kyuichi Taguma1, Atsushi Yoshiki1, Atsuo Ogura1 1RIKEN BioResource Center Uma etileno glicol baseado em método de vitrificação para embriões de camundongos é descrito. É vantajoso para outros métodos, em sua simplicidade e toxicidade embrionária baixo e, portanto, pode ser amplamente aplicável a muitas cepas de camundongos, incluindo camundongos isogênicos e gene modificado.
Other articles by Kyuichi Taguma on PubMed
An Optimal Embryo Transfer Condition for the Effective Production of DBA/2J Mice Experimental Animals / Japanese Association for Laboratory Animal Science. Oct, 2007 | Pubmed ID: 18075200 The DBA/2J mouse strain is a standard laboratory strain that is widely used for biomedical research. This strain, however, suffers from poor reproductive performance. In addition, the conditions for reliable embryo transfer (ET) of this strain have not been elucidated. The intention of this study was to determine the optimal number of embryos for transfer that allow the effective production of DBA/2J offspring. In the experiment, 7 to 15 embryos per oviduct were transferred into pseudopregnant ICR females. A relatively high success rate for pup production was observed when a large number of DBA/2J embryos (30 embryos per female) were transferred. This result shows that the ET efficiency of the DBA/2J strain can be improved by increasing the number of transferred embryos.
A Practical Novel Method for Ensuring Stable Capacitation of Spermatozoa from Cryopreserved C57BL/6J Sperm Suspension Experimental Animals / Japanese Association for Laboratory Animal Science. Jul, 2009 | Pubmed ID: 19654437 A large number of genetically modified mouse strains have been produced in recent years. Sperm cryopreservation is the most effective means of preserving these valuable strains, most of which have a C57BL/6 genetic background. However, the fertilization efficiency of sperm from several cryopreserved strains, including C57BL/6, is quite low. While new and improved methods of cryopreservation have been developed, the majority of sperm stocks have already been cryopreserved using traditional methods, such as storage in 18% raffinose and 3% skim milk (R18S3). Therefore, new thawing methods for these frozen stocks are needed. We have developed a new thawing method that involves selective collection of motile sperm and a preincubation medium that enhances capacitation. Motile sperm are selected simply by collecting a sample from the center of a dish, and capacitation is induced by the addition of methyl-beta-cyclodextrin, D-penicillamine, sodium citrate, and hypotaurine to modified Tyrode's solution. The fertilization rate of sperm prepared using this method was increased significantly compared to that of sperm thawed using the traditional method (63.9 vs 16.5%, P