Articles by Lars Roed Ingerslev in JoVE
Methodology for Accurate Detection of Mitochondrial DNA Methylation Mie Mechta1, Lars Roed Ingerslev1, Romain Barrès1 1The Novo Nordisk Foundation for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen Here, we present a protocol to allow accurate quantification of mitochondrial DNA (mtDNA) methylation. In this protocol, we describe an enzymatic digestion of DNA with BamHI coupled with a bioinformatic analysis pipeline which can be used to avoid overestimation of mtDNA methylation levels caused by the secondary structure of mtDNA.
Other articles by Lars Roed Ingerslev on PubMed
In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells Cell Reports. | Pubmed ID: 29141227 State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation steps followed by flow cytometry, exposing cells to stress and disrupting their physiological niche. Focusing on adult skeletal muscle stem cells, we have developed a protocol that circumvents the impact of isolation procedures and captures cells in their native quiescent state. We show that current isolation protocols induce major transcriptional changes accompanied by specific histone modifications while having negligible effects on DNA methylation. In addition to proposing a protocol to avoid isolation-induced artifacts, our study reveals previously undetected quiescence and early activation genes of potential biological interest.
Preadipocytes from Obese Humans with Type 2 Diabetes Are Epigenetically Reprogrammed at Genes Controlling Adipose Tissue Function International Journal of Obesity (2005). | Pubmed ID: 29511320 Deterioration of the adipogenic potential of preadipocytes may contribute to adipose tissue dysfunction in obesity and type 2 diabetes (T2D). Here, we hypothesized that extracellular factors in obesity epigenetically reprogram adipogenesis potential and metabolic function of preadipocytes.