Other Publications (1)
Articles by Latifa Bakiri in JoVE
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration Maria Riedel1, Martin F. Berthelsen1, Latifa Bakiri2, Erwin F. Wagner2, Martin K. Thomsen1 1Department of Clinical Medicine, Aarhus University, 2GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO) This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer.
Other articles by Latifa Bakiri on PubMed
Mouse Models for Liver Cancer Molecular Oncology. | Pubmed ID: 23428636 Hepatocellular carcinoma (HCC), the most common form of primary liver cancer is the third leading cause of cancer-related cell death in human and the fifth in women worldwide. The incidence of HCC is increasing despite progress in identifying risk factors, understanding disease etiology and developing anti-viral strategies. Therapeutic options are limited and survival after diagnosis is poor. Therefore, better preventive, diagnostic and therapeutic tools are urgently needed, in particular given the increased contribution from systemic metabolic disease to HCC incidence worldwide. In the last three decades, technological advances have facilitated the generation of genetically engineered mouse models (GEMMs) to mimic the alterations frequently observed in human cancers or to conduct intervention studies and assess the relevance of candidate gene networks in tumor establishment, progression and maintenance. Because these studies allow molecular and cellular manipulations impossible to perform in patients, GEMMs have improved our understanding of this complex disease and represent a source of great potential for mechanism-based therapy development. In this review, we provide an overview of the current state of HCC modeling in the mouse, highlighting successes, current challenges and future opportunities.