In JoVE (1)
Other Publications (13)
- Journal of Cancer Education : the Official Journal of the American Association for Cancer Education
- Sensors (Basel, Switzerland)
- Journal of Acquired Immune Deficiency Syndromes (1999)
- PloS One
- Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
- International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
- AJR. American Journal of Roentgenology
- Microporous and Mesoporous Materials : the Official Journal of the International Zeolite Association
- Journal of Lesbian Studies
- Disease Models & Mechanisms
- CNS & Neurological Disorders Drug Targets
Articles by Laura Jimenez in JoVE
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response Mattie J. Casey*1, Katarzyna Modzelewska*1, Daniela Anderson1, James Goodman1, Elena F. Boer1, Laura Jimenez1, Douglas Grossman1,2, Rodney A. Stewart1 1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
Other articles by Laura Jimenez on PubMed
Progress in the Pipeline: Replication of the Minority Training Program in Cancer Control Research Journal of Cancer Education : the Official Journal of the American Association for Cancer Education. 2006 | Pubmed ID: 17542715 This paper evaluates the replicability of an NCI-funded didactic/experiential program to increase the diversity of doctorally-prepared cancer disparities investigators.
Analysis of Electromyographic Signals from Rats' Stomaches for Detection and Classification of Motility Sensors (Basel, Switzerland). May, 2008 | Pubmed ID: 27879860 This paper presents the analysis of the electromyographic signals from rat stomaches to identify and classify contractions. The results were validated with both visual identification and an ultrasonic system to guarantee the reference. Some parameters were defined and associated to the energy of the signal in frequency domain and grouped in a P vector. The parameters were statistically analyzed and according to the results, an artificial neuronal network was designed to use the P vectors as inputs to classify the electrical signals related to the contraction conditions. A first approach classification was performed with and without contraction classes (CR and NCR), then the same database were subdivided in four classes: with induced contraction (ICR), spontaneous contraction (SCR), without contraction due a post mortem condition (PMR) or under physiological conditions (PNCR). In a two-class classifier, performance was 86%, 93% and 91% of detections for each electrogastromyografic (EGMG) signal from each of three pairs of electrodes considered. Because in the four-class classifier, enough data was not collected for the first pair, then a three-class classifier with 82% of performance was used. For the other two EGMG signals electrode pairs, performance was of 76% and 86% respectively. Based in the results, the analysis of P vectors could be used as a contraction detector in motility studies due to different stimuli in a rat model.
HIV-1 Transmission Cluster with T215D Revertant Mutation Among Newly Diagnosed Patients from the Basque Country, Spain Journal of Acquired Immune Deficiency Syndromes (1999). May, 2009 | Pubmed ID: 19282784 To determine the introduction of HIV-1 genetic forms and to examine transmission clusters and resistance to antiretroviral inhibitors among newly diagnosed patients from the Basque Country, Spain, during 2004-2007.
Encouraging Expressions Affect the Brain and Alter Visual Attention PloS One. Jun, 2009 | Pubmed ID: 19536283 Very often, encouraging or discouraging expressions are used in competitive contexts, such as sports practice, aiming at provoking an emotional reaction on the listener and, consequently, an effect on subsequent cognition and/or performance. However, the actual efficiency of these expressions has not been tested scientifically.
Molecular Mechanisms Behind the Resistance of Cisplatin in Germ Cell Tumours Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico. Dec, 2009 | Pubmed ID: 20045784 Cisplatin has been one of the principal chemotherapy agents for the last 30 years and is still used widely in the treatment of testicular, ovarian, lung, head and neck, bladder and several other tumours. Resistance to chemotherapeutic agents is a major obstacle for successful treatment. Treatment effect on germ cell tumours (GCTs) is more successful than in adults suffering from almost any other solid tumour, but resistance still appears in 20% of patients with metastatic disease. However, because of the young age of patients and few data regarding the process of becoming resistant, this situation is still a challenge. In this review we are going to analyse the published literature on cisplatin resistance in GCTs and explain the initiatives that the Spanish Germ Cell Cancer Group (GG) is taking to try to elucidate the molecular mechanisms behind this process.
CA-125 Response Patterns in Patients with Recurrent Ovarian Cancer Treated with Pegylated Liposomal Doxorubicin (PLD) International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society. Jan, 2010 | Pubmed ID: 20130507 : In recurrent ovarian cancer, CA-125 could be the only objective response criteria. This study analyzes response patterns regarding CA-125 in responders versus nonresponders and determines whether a specific cutoff value for CA-125 could predict clinical response, compared with response evaluation criteria in solid tumors, in patients receiving pegylated liposomal doxorubicin (PLD).
Image Quality and Radiation Dose of Pulmonary CT Angiography Performed Using 100 and 120 KVp AJR. American Journal of Roentgenology. Nov, 2012 | Pubmed ID: 23096170 The objective of our study was to compare image quality and radiation dose of pulmonary CT angiography (CTA) performed in the same patient cohort using tube potentials of 100 and 120 kVp.
The Use of Lanthanide Luminescence As a Reporter in the Solid State: Desymmetrization of the Prochiral Layers of γ-zirconium Phosphate/phosphonate and Circularly Polarized Luminescence Microporous and Mesoporous Materials : the Official Journal of the International Zeolite Association. Mar, 2013 | Pubmed ID: 23329880 Solid-state CPL measurements were performed for the first time on hybrid, laminar materials based on γ-ZrP pillared with organic diphosphonates. Ad hoc optically pure diphosphonates were synthesized and the luminescence properties of their complexation with Tb(III) were verified in solution. CD and CPL measurements showed that the bistriazolylpyridine chromophores bonded to the metal provided an effective chiral environment that produced significant signals. In the case of the γ-ZrP-derived materials, experimental evidence and simple molecular modeling hinted to the occurrence of supramolecular chirality in the particles, induced by the intrinsic dissymmetry of the organic diphosphonates or by the intercalation of chiral species such as 1-phenethylamine. Chirality at the supramolecular level was revealed in the solid state by the CPL signals measured from reporter Tb(III) ions intercalated in the hybrid matrix.
Representations in Award-Winning LGBTQ Young Adult Literature from 2000-2013 Journal of Lesbian Studies. 2015 | Pubmed ID: 26264988 Lesbian, gay, bisexual, transgendered, and queer (LGBTQ) young adult (YA) literature is increasing in popularity, with novels like Bil Wright's Putting Makeup on the Fat Boy winning the two LGBTQ YA honors--the Lambda Literary and Stonewall Book Awards--as well awards commending their cultural diversity. Despite the upsurge of celebrated LGBTQ YA literature, a study of the protagonists in Lambda- and Stonewall-winning YA novels from 2000-2013 reveals three findings: the dominance of White, gay, male characters contradicts the trend toward strong female protagonists in mainstream YA; stories about lesbians are primarily tragic; and there are no bisexual protagonists.
CD69 Expression Potentially Predicts Response to Bendamustine and Its Modulation by Ibrutinib or Idelalisib Enhances Cytotoxic Effect in Chronic Lymphocytic Leukemia Oncotarget. Feb, 2016 | Pubmed ID: 26701728 Clinical responses to bendamustine in chronic lymphocytic leukemia (CLL) are highly heterogeneous and no specific markers to predict sensitivity to this drug have been reported. In order to identify biomarkers of response, we analyzed the in vitro activity of bendamustine and the gene expression profile in primary CLL cells. We observed that mRNA expression of CD69 (CD69) and ITGAM (CD11b) constitute the most powerful predictor of response to bendamustine. When we interrogated the predictive value of the corresponding cell surface proteins, the expression of the activation marker CD69 was the most reliable predictor of sensitivity to bendamustine. Importantly, a multivariate analysis revealed that the predictive value of CD69 expression was independent from other clinico-biological CLL features. We also showed that when CLL cells were co-cultured with distinct subtypes of stromal cells, an upregulation of CD69 was accompanied by a reduced sensitivity to bendamustine. In agreement with this, tumor cells derived from lymphoid tumor niches harbored higher CD69 expression and were less sensitive to bendamustine than their peripheral blood counterparts. Furthermore, pretreatment of CD69 high CLL cases with the B-cell receptor (BCR) pathway inhibitors ibrutinib and idelalisib decreased CD69 levels and enhanced bendamustine cytotoxic effect. Collectively, our findings indicate that CD69 could be a predictor of bendamustine response in CLL patients and the combination of clinically-tested BCR signaling inhibitors with bendamustine may represent a promising strategy for bendamustine low responsive CLL cases.
Phenotypic Chemical Screening Using a Zebrafish Neural Crest EMT Reporter Identifies Retinoic Acid As an Inhibitor of Epithelial Morphogenesis Disease Models & Mechanisms. Apr, 2016 | Pubmed ID: 26794130 The epithelial-to-mesenchymal transition (EMT) is a highly conserved morphogenetic program essential for embryogenesis, regeneration and cancer metastasis. In cancer cells, EMT also triggers cellular reprogramming and chemoresistance, which underlie disease relapse and decreased survival. Hence, identifying compounds that block EMT is essential to prevent or eradicate disseminated tumor cells. Here, we establish a whole-animal-based EMT reporter in zebrafish for rapid drug screening, calledTg(snai1b:GFP), which labels epithelial cells undergoing EMT to producesox10-positive neural crest (NC) cells. Time-lapse and lineage analysis ofTg(snai1b:GFP)embryos reveal that cranial NC cells delaminate from two regions: an early population delaminates adjacent to the neural plate, whereas a later population delaminates from within the dorsal neural tube. TreatingTg(snai1b:GFP)embryos with candidate small-molecule EMT-inhibiting compounds identified TP-0903, a multi-kinase inhibitor that blocked cranial NC cell delamination in both the lateral and medial populations. RNA sequencing (RNA-Seq) analysis and chemical rescue experiments show that TP-0903 acts through stimulating retinoic acid (RA) biosynthesis and RA-dependent transcription. These studies identify TP-0903 as a new therapeutic for activating RAin vivoand raise the possibility that RA-dependent inhibition of EMT contributes to its prior success in eliminating disseminated cancer cells.
Neuroprotective Effects of Cannabidiol in Hypoxic Ischemic Insult. The Therapeutic Window in Newborn Mice CNS & Neurological Disorders Drug Targets. 2017 | Pubmed ID: 27686886 A relevant therapeutic time window (TTW) is an important criterion for considering the clinical relevance of a substance preventing newborn hypoxic-ischemic (HI) brain damage.