In JoVE (1)
Other Publications (1)
Articles by Laurence Buisseret in JoVE
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes Soizic Garaud*1,2, Chunyan Gu-Trantien*1,2, Jean-Nicolas Lodewyckx1,2, Anaïs Boisson1,2, Pushpamali De Silva1,2, Laurence Buisseret1,2, Edoardo Migliori1,2, Myriam Libin3, Céline Naveaux1,2, Hugues Duvillier1,4, Karen Willard-Gallo1,2 1Molecular Immunology Unit, Université Libre de Bruxelles, 2Institut Jules Bordet, Université Libre de Bruxelles, 3Institut d'Immunologie Médicale, Université Libre de Bruxelles, 4Flow Cytometry Core Facility, Université Libre de Bruxelles This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.
Other articles by Laurence Buisseret on PubMed
Optic Neuropathy, Renal Failure and Pulmonary Sarcoidosis in a 50-year-old Man: Where is the Link? BMJ Case Reports. 2009 | Pubmed ID: 21931581 Eye disorders are frequently associated with renal diseases, mostly linked to underlying causes such as hypertension, diabetes or autoimmune diseases. Conversely, advanced uraemic states may also lead to progressive vision impairment. The present report concerns a 50-year-old patient who presented with a bilateral, painless, progressive vision loss, a moderate systemic inflammation and chronic renal failure due to hypertension nephrosclerosis. Steroids were given and haemodialysis was initiated, resulting in vision improvement. At 4 months later when the steroids were stopped, the patient developed dyspnoea, cough, fever and fatigue of unclear origin. A lung biopsy showed non-caseating granuloma consistent with pulmonary sarcoidosis. Re-challenge with steroids rapidly improved the respiratory disease. Ophthalmological examinations performed early and later in the course excluded anterior ischaemic optic neuropathy and ocular manifestations of sarcoidosis, leading to a diagnosis of uraemic optic neuropathy. This rare ophthalmological disorder should be promptly recognised since haemodialysis and steroid therapy are highly effective.