Articles by Leah C Acker in JoVE
Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates Leah C Acker1,2,3, Erica N. Pino1,4,5, Edward S. Boyden1,6,7, Robert Desimone1,7 1McGovern Institute, Massachusetts Institute of Technology, 2Harvard-MIT Division of Heath Sciences and Technology, 3Department of Anesthesiology, Duke University Medical Center, 4Department of Biology, Massachusetts Institute of Technology, 5Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, 6Media Lab and Department of Biological Engineering, Massachusetts Institute of Technology, 7Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology A protocol to build a tissue penetrating illuminator for delivering light over large volumes with minimal diameter is presented.
Other articles by Leah C Acker on PubMed
Semiautomatic Vessel Wall Detection and Quantification of Wall Thickness in Computed Tomography Images of Human Abdominal Aortic Aneurysms Medical Physics. Feb, 2010 | Pubmed ID: 20229873 Quantitative measurements of wall thickness in human abdominal aortic aneurysms (AAAs) may lead to more accurate methods for the evaluation of their biomechanical environment.
Noninvasive Optical Inhibition with a Red-shifted Microbial Rhodopsin Nature Neuroscience. Aug, 2014 | Pubmed ID: 24997763 Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.