Leonid Margolis Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development National Institutes of Health Biography Publications Institution JoVE Articles Leonid Margolis has not added a biography. If you are Leonid Margolis and would like to personalize this page please email our Author Liaison for assistance. Publications Monocytes of Different Subsets in Complexes with Platelets in Patients with Myocardial Infarction Thrombosis and Haemostasis. Oct, 2018 | Pubmed ID: 30300910 Human Cytomegalovirus-infected Cells Release Extracellular Vesicles That Carry Viral Surface Proteins Virology. 11, 2018 | Pubmed ID: 30165311 A System of Cytokines Encapsulated in ExtraCellular Vesicles Scientific Reports. Jun, 2018 | Pubmed ID: 29895824 Extracellular Vesicles Generated by Placental Tissues Ex Vivo: A Transport System for Immune Mediators and Growth Factors American Journal of Reproductive Immunology (New York, N.Y. : 1989). Jul, 2018 | Pubmed ID: 29726582 Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam Methods in Molecular Biology (Clifton, N.J.). 2018 | Pubmed ID: 29572804 CD63, MHC Class 1, and CD47 Identify Subsets of Extracellular Vesicles Containing Distinct Populations of Noncoding RNAs Scientific Reports. Feb, 2018 | Pubmed ID: 29416092 Ex vivo Culture of Human Atherosclerotic Plaques: A Model to Study Immune Cells in Atherogenesis Atherosclerosis. Dec, 2017 | Pubmed ID: 29101840 Virtual Screening of Acyclovir Derivatives As Potential Antiviral Agents: Design, Synthesis, and Biological Evaluation of New Acyclic Nucleoside ProTides Journal of Medicinal Chemistry. Sep, 2017 | Pubmed ID: 28829913 Dual-targeted Anti-TB/anti-HIV Heterodimers Antiviral Research. Sep, 2017 | Pubmed ID: 28743447 Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine Cell Host & Microbe. Jul, 2017 | Pubmed ID: 28704658 A Common Anti-cytomegalovirus Drug, Ganciclovir, Inhibits HIV-1 Replication in Human Tissues Ex Vivo AIDS (London, England). 07, 2017 | Pubmed ID: 28657962 The Cytokine Network in Women with an Asymptomatic Short Cervix and the Risk of Preterm Delivery American Journal of Reproductive Immunology (New York, N.Y. : 1989). Sep, 2017 | Pubmed ID: 28585708 Vaginal Inhibits HIV-1 Replication in Human Tissues Frontiers in Microbiology. 2017 | Pubmed ID: 28579980 Extracellular Vesicles Carry HIV Env and Facilitate Hiv Infection of Human Lymphoid Tissue Scientific Reports. May, 2017 | Pubmed ID: 28490736 Flow Virometry Analysis of Envelope Glycoprotein Conformations on Individual HIV Virions Scientific Reports. 04, 2017 | Pubmed ID: 28424455 Addition of Thrombin Reduces the Recovery of Extracellular Vesicles from Blood Plasma Journal of Circulating Biomarkers. Jan-Dec, 2016 | Pubmed ID: 28936260 Flow Analysis of Individual Blood Extracellular Vesicles in Acute Coronary Syndrome Platelets. Sep, 2016 | Pubmed ID: 27595614 Monocytes but Not Lymphocytes Carrying HIV-1 on Their Surface Transmit Infection to Human Tissue Ex Vivo Journal of Virology. Nov, 2016 | Pubmed ID: 27558419 Cytomegalovirus-Productive Infection Is Associated With Acute Coronary Syndrome Journal of the American Heart Association. Aug, 2016 | Pubmed ID: 27543799 Modulation of Immune Responses by Extracellular Vesicles From Retinal Pigment Epithelium Investigative Ophthalmology & Visual Science. Aug, 2016 | Pubmed ID: 27537259 Extracellular Vesicles and Viruses: Are They Close Relatives? Proceedings of the National Academy of Sciences of the United States of America. Aug, 2016 | Pubmed ID: 27432966 Systemic Inflammation and Liver Damage in HIV/hepatitis C Virus Coinfection HIV Medicine. 09, 2016 | Pubmed ID: 27187749 Evaluation of the Maturation of Individual Dengue Virions with Flow Virometry Virology. Jan, 2016 | Pubmed ID: 26590794 Distinct Cytokine/chemokine Network in Semen and Blood Characterize Different Stages of HIV Infection AIDS (London, England). Jan, 2016 | Pubmed ID: 26558730 Evidence of Perturbations of the Cytokine Network in Preterm Labor American Journal of Obstetrics and Gynecology. Dec, 2015 | Pubmed ID: 26232508 Genital Cytomegalovirus Replication Predicts Syphilis Acquisition Among HIV-1 Infected Men Who Have Sex with Men PloS One. 2015 | Pubmed ID: 26061824 Valacyclovir Decreases Plasma HIV-1 RNA in HSV-2 Seronegative Individuals: A Randomized Placebo-Controlled Crossover Trial Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America. Jun, 2015 | Pubmed ID: 25740794 Immunoactivation at the Crossroads of Human Disease The American Journal of Medicine. Jun, 2015 | Pubmed ID: 25637756 Antigenic Composition of Single Nano-sized Extracellular Blood Vesicles Nanomedicine : Nanotechnology, Biology, and Medicine. Apr, 2015 | Pubmed ID: 25481806 An Ex Vivo Model of HIV-1 Infection in Human Lymphoid Tissue and Cervico-vaginal Tissue Bio-protocol. 2014 | Pubmed ID: 25774380 A New Antiviral: Chimeric 3TC-AZT Phosphonate Efficiently Inhibits HIV-1 in Human Tissues Ex Vivo Antiviral Research. Sep, 2014 | Pubmed ID: 25010891 HIV-1 Vaginal Transmission: Cell-free or Cell-associated Virus? American Journal of Reproductive Immunology (New York, N.Y. : 1989). Jun, 2014 | Pubmed ID: 24730358 Histocultures (tissue Explants) in Human Retrovirology Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24158827 Design, Synthesis and Biological Evaluation of Phosphorodiamidate Prodrugs of Antiviral and Anticancer Nucleosides European Journal of Medicinal Chemistry. 2013 | Pubmed ID: 24177359 A Multi-targeted Drug Candidate with Dual Anti-HIV and Anti-HSV Activity PLoS Pathogens. 2013 | Pubmed ID: 23935482 Nanoparticle-based Flow Virometry for the Analysis of Individual Virions The Journal of Clinical Investigation. Sep, 2013 | Pubmed ID: 23925291 Interleukin-7 Facilitates HIV-1 Transmission to Cervico-vaginal Tissue Ex Vivo PLoS Pathogens. Feb, 2013 | Pubmed ID: 23408885 Productive HIV-1 Infection of Human Cervical Tissue Ex Vivo is Associated with the Secretory Phase of the Menstrual Cycle Mucosal Immunology. Nov, 2013 | Pubmed ID: 23385427 It Takes More Than Two for This Tango Cell Host & Microbe. Jan, 2013 | Pubmed ID: 23332149 HIV-1 Expressing the Envelopes of Transmitted/founder or Control/reference Viruses Have Similar Infection Patterns of CD4 T-cells in Human Cervical Tissue Ex Vivo PloS One. 2012 | Pubmed ID: 23236398 HIV-1 Imposes Rigidity on Blood and Semen Cytokine Networks American Journal of Reproductive Immunology (New York, N.Y. : 1989). Dec, 2012 | Pubmed ID: 23006048 Microbicides: Still a Long Road to Success Trends in Microbiology. Aug, 2012 | Pubmed ID: 22705107 HIV-1 is Not a Major Driver of Increased Plasma IL-6 Levels in Chronic HIV-1 Disease Journal of Acquired Immune Deficiency Syndromes (1999). Oct, 2012 | Pubmed ID: 22659649 Exploiting the Anti-HIV-1 Activity of Acyclovir: Suppression of Primary and Drug-resistant HIV Isolates and Potentiation of the Activity by Ribavirin Antimicrobial Agents and Chemotherapy. May, 2012 | Pubmed ID: 22314523 Semen of HIV-1-infected Individuals: Local Shedding of Herpesviruses and Reprogrammed Cytokine Network The Journal of Infectious Diseases. Jan, 2012 | Pubmed ID: 22107749 Topical Tenofovir, a Microbicide Effective Against HIV, Inhibits Herpes Simplex Virus-2 Replication Cell Host & Microbe. Oct, 2011 | Pubmed ID: 22018238 Activation of T Lymphocytes in Atherosclerotic Plaques Arteriosclerosis, Thrombosis, and Vascular Biology. Dec, 2011 | Pubmed ID: 21960562 CD4+ T Cells, Including Th17 and Cycling Subsets, Are Intact in the Gut Mucosa of HIV-1-infected Long-term Nonprogressors Journal of Virology. Jun, 2011 | Pubmed ID: 21471231 Selective Transmission of R5 HIV-1 Variants: Where is the Gatekeeper? Journal of Translational Medicine. Jan, 2011 | Pubmed ID: 21284905 Cervico-vaginal Tissue Ex Vivo As a Model to Study Early Events in HIV-1 Infection American Journal of Reproductive Immunology (New York, N.Y. : 1989). Mar, 2011 | Pubmed ID: 21223429 Determinants of Protection Among HIVâ€exposed Seronegative Persons: an Overview The Journal of Infectious Diseases. Nov, 2010 | Pubmed ID: 20887220 Contrasting Roles for TLR Ligands in HIV-1 Pathogenesis PloS One. Sep, 2010 | Pubmed ID: 20862220 HIV-1 Sexual Transmission: Early Events of HIV-1 Infection of Human Cervico-vaginal Tissue in an Optimized Ex Vivo Model Mucosal Immunology. May, 2010 | Pubmed ID: 20147895 A New Class of Dual-targeted Antivirals: Monophosphorylated Acyclovir Prodrug Derivatives Suppress Both Human Immunodeficiency Virus Type 1 and Herpes Simplex Virus Type 2 The Journal of Infectious Diseases. Feb, 2010 | Pubmed ID: 20085496 War and Peace Between Microbes: HIV-1 Interactions with Coinfecting Viruses Cell Host & Microbe. Nov, 2009 | Pubmed ID: 19917495 Acyclovir: a New Use for an Old Drug Current Opinion in Infectious Diseases. Dec, 2009 | Pubmed ID: 19726982 Multisite Comparison of Anti-human Immunodeficiency Virus Microbicide Activity in Explant Assays Using a Novel Endpoint Analysis Journal of Clinical Microbiology. Nov, 2009 | Pubmed ID: 19726602 Movements of HIV-virions in Human Cervical Mucus Biomacromolecules. Sep, 2009 | Pubmed ID: 19711976 A Missed Point in Deciphering the Viral Synergy Between Herpes Simplex Virus and HIV The Lancet. Infectious Diseases. Sep, 2009 | Pubmed ID: 19695487 Toxoplasma Gondii Inhibits R5 HIV-1 Replication in Human Lymphoid Tissues Ex Vivo Microbes and Infection. Dec, 2009 | Pubmed ID: 19671446 The Application of Phosphoramidate Protide Technology to Acyclovir Confers Anti-HIV Inhibition Journal of Medicinal Chemistry. Sep, 2009 | Pubmed ID: 19645484 Immune Suppression of Human Lymphoid Tissues and Cells in Rotating Suspension Culture and Onboard the International Space Station In Vitro Cellular & Developmental Biology. Animal. Dec, 2009 | Pubmed ID: 19609626 Evolution of SIV Toward RANTES Resistance in Macaques Rapidly Progressing to AIDS Upon Coinfection with HHV-6A Retrovirology. Jul, 2009 | Pubmed ID: 19573243 Measurement of Human Immunodeficiency Virus Type 1 Preintegration Transcription by Using Rev-dependent Rev-CEM Cells Reveals a Sizable Transcribing DNA Population Comparable to That from Proviral Templates Journal of Virology. Sep, 2009 | Pubmed ID: 19553325 Mechanisms Associated with HIV-1 Resistance to Acyclovir by the V75I Mutation in Reverse Transcriptase The Journal of Biological Chemistry. Aug, 2009 | Pubmed ID: 19509419 Genetic Variation in OAS1 is a Risk Factor for Initial Infection with West Nile Virus in Man PLoS Pathogens. Feb, 2009 | Pubmed ID: 19247438 Use of Human Tissue Explants to Study Human Infectious Agents Nature Protocols. 2009 | Pubmed ID: 19197269 Integrating Integrins into HIV Pathogenesis Blood. Feb, 2009 | Pubmed ID: 19196869 Coinfecting Viruses As Determinants of HIV Disease Current HIV/AIDS Reports. Feb, 2009 | Pubmed ID: 19149991 Unsung Hero Robert C. Gallo Science (New York, N.Y.). Jan, 2009 | Pubmed ID: 19131607 A Highly Sensitive and Dynamic Immunofluorescent Cytometric Bead Assay for the Detection of HIV-1 P24 Journal of Virological Methods. Apr, 2009 | Pubmed ID: 19100289 Acyclovir is Activated into a HIV-1 Reverse Transcriptase Inhibitor in Herpesvirus-infected Human Tissues Cell Host & Microbe. Sep, 2008 | Pubmed ID: 18779052 The Lymph Node in HIV Pathogenesis Seminars in Immunology. Jun, 2008 | Pubmed ID: 18620868 Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: a Multicenter Study Analytical Chemistry. Jun, 2008 | Pubmed ID: 18484740 Upregulation of Human Cytomegalovirus by HIV Type 1 in Human Lymphoid Tissue Ex Vivo AIDS Research and Human Retroviruses. Mar, 2008 | Pubmed ID: 18327985 HIV-1 Induced Activation of CD4+ T Cells Creates New Targets for HIV-1 Infection in Human Lymphoid Tissue Ex Vivo Blood. Jan, 2008 | Pubmed ID: 17909079 Semen-derived Amyloid Fibrils Drastically Enhance HIV Infection Cell. Dec, 2007 | Pubmed ID: 18083097 Sustained Secretion of Immunoglobulin by Long-lived Human Tonsil Plasma Cells The American Journal of Pathology. Sep, 2007 | Pubmed ID: 17690187 Measles Virus Vaccine Attenuation: Suboptimal Infection of Lymphatic Tissue and Tropism Alteration The Journal of Infectious Diseases. Aug, 2007 | Pubmed ID: 17624839 Design and Cellular Kinetics of Dansyl-labeled CADA Derivatives with Anti-HIV and CD4 Receptor Down-modulating Activity Biochemical Pharmacology. Aug, 2007 | Pubmed ID: 17603023 HIV-1 Pathogenesis Differs in Rectosigmoid and Tonsillar Tissues Infected Ex Vivo with CCR5- and CXCR4-tropic HIV-1 AIDS (London, England). Jun, 2007 | Pubmed ID: 17545702 Cell-cell and Cell-extracellular Matrix Interactions Regulate Embryonic Stem Cell Differentiation Stem Cells (Dayton, Ohio). Mar, 2007 | Pubmed ID: 17332514 Abnormal Activation and Cytokine Spectra in Lymph Nodes of People Chronically Infected with HIV-1 Blood. May, 2007 | Pubmed ID: 17289812 Evolution of CXCR4-using Human Immunodeficiency Virus Type 1 SF162 is Associated with Two Unique Envelope Mutations Journal of Virology. Apr, 2007 | Pubmed ID: 17202224 Viral Interactions in Human Lymphoid Tissue: Human Herpesvirus 7 Suppresses the Replication of CCR5-tropic Human Immunodeficiency Virus Type 1 Via CD4 Modulation Journal of Virology. Jan, 2007 | Pubmed ID: 17065205 Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-dimensional Collagen Matrix Methods in Molecular Biology (Clifton, N.J.). 2006 | Pubmed ID: 16846041 Ex Vivo Culture of Human Colorectal Tissue for the Evaluation of Candidate Microbicides AIDS (London, England). Jun, 2006 | Pubmed ID: 16816551 Selective Transmission of CCR5-utilizing HIV-1: the 'gatekeeper' Problem Resolved? Nature Reviews. Microbiology. Apr, 2006 | Pubmed ID: 16541138 The Nonnucleoside Reverse Transcriptase Inhibitor UC-781 Inhibits Human Immunodeficiency Virus Type 1 Infection of Human Cervical Tissue and Dissemination by Migratory Cells Journal of Virology. Sep, 2005 | Pubmed ID: 16103169 Differential Pathogenesis of Primary CCR5-using Human Immunodeficiency Virus Type 1 Isolates in Ex Vivo Human Lymphoid Tissue Journal of Virology. Sep, 2005 | Pubmed ID: 16103166 Pertussis Toxin B-oligomer Dissociates T Cell Activation and HIV Replication in CD4 T Cells Released from Infected Lymphoid Tissue AIDS (London, England). Jul, 2005 | Pubmed ID: 15958831 Inhibition of HIV-1 Replication in Human Lymphoid Tissues Ex Vivo by Measles Virus The Journal of Infectious Diseases. Jul, 2005 | Pubmed ID: 15942896 Invasion of Human Tissue Ex Vivo by Borrelia Burgdorferi The Journal of Infectious Diseases. May, 2005 | Pubmed ID: 15838803 Complex Extracellular Matrices Promote Tissue-specific Stem Cell Differentiation Stem Cells (Dayton, Ohio). Feb, 2005 | Pubmed ID: 15671151 Vpr and Vpu Are Important for Efficient Human Immunodeficiency Virus Type 1 Replication and CD4+ T-cell Depletion in Human Lymphoid Tissue Ex Vivo Journal of Virology. Nov, 2004 | Pubmed ID: 15507658 Depletion of CD4 T Lymphocytes in Human Lymphoid Tissue Infected Ex Vivo with Doxycycline-dependent HIV-1 Virology. Oct, 2004 | Pubmed ID: 15380352 Dual Role of Prostratin in Inhibition of Infection and Reactivation of Human Immunodeficiency Virus from Latency in Primary Blood Lymphocytes and Lymphoid Tissue Journal of Virology. Oct, 2004 | Pubmed ID: 15367617 A Naturally Occurring Variation in the Proline-rich Region Does Not Attenuate Human Immunodeficiency Virus Type 1 Nef Function Journal of Virology. Sep, 2004 | Pubmed ID: 15331753 Noninfectious X4 but Not R5 Human Immunodeficiency Virus Type 1 Virions Inhibit Humoral Immune Responses in Human Lymphoid Tissue Ex Vivo Journal of Virology. Jul, 2004 | Pubmed ID: 15194782 CXCR4-tropic HIV-1 Suppresses Replication of CCR5-tropic HIV-1 in Human Lymphoid Tissue by Selective Induction of CC-chemokines The Journal of Infectious Diseases. Feb, 2004 | Pubmed ID: 14745709 Membrane Cholesterol, Lateral Mobility, and the Phosphatidylinositol 4,5-bisphosphate-dependent Organization of Cell Actin Proceedings of the National Academy of Sciences of the United States of America. Nov, 2003 | Pubmed ID: 14612561 Pathogenic Effects of Human Herpesvirus 6 in Human Lymphoid Tissue Ex Vivo Journal of Virology. Aug, 2003 | Pubmed ID: 12857897 Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture Stem Cells (Dayton, Ohio). 2003 | Pubmed ID: 12832701 Multilineage Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-dimensional Culture Systems Stem Cells (Dayton, Ohio). 2003 | Pubmed ID: 12743323 Real-time PCR Assay of Individual Human Immunodeficiency Virus Type 1 Variants in Coinfected Human Lymphoid Tissues Journal of Clinical Microbiology. May, 2003 | Pubmed ID: 12734258 Bystander CD4+ T Lymphocytes Survive in HIV-infected Human Lymphoid Tissue AIDS Research and Human Retroviruses. Mar, 2003 | Pubmed ID: 12689413 Cytokines--strategic Weapons in Germ Warfare? Nature Biotechnology. Jan, 2003 | Pubmed ID: 12511900 Segregation of R5 and X4 HIV-1 Variants to Memory T Cell Subsets Differentially Expressing CD62L in Ex Vivo Infected Human Lymphoid Tissue AIDS (London, England). Jun, 2002 | Pubmed ID: 12045489 Human Immunodeficiency Virus Type 1 (HIV-1) Non-B Subtypes Are Similar to HIV-1 Subtype B in That Coreceptor Specificity is a Determinant of Cytopathicity in Human Lymphoid Tissue Infected Ex Vivo Journal of Virology. Nov, 2001 | Pubmed ID: 11581424 CD4 Down-modulation by Human Immunodeficiency Virus Type 1 Nef Correlates with the Efficiency of Viral Replication and with CD4(+) T-cell Depletion in Human Lymphoid Tissue Ex Vivo Journal of Virology. Nov, 2001 | Pubmed ID: 11581379 Human Immunodeficiency Virus Type 1 Induces Apoptosis in CD4(+) but Not in CD8(+) T Cells in Ex Vivo-infected Human Lymphoid Tissue Journal of Virology. Sep, 2000 | Pubmed ID: 10933717 Human Immunodeficiency Virus Type 1 Coreceptor Preferences Determine Target T-cell Depletion and Cellular Tropism in Human Lymphoid Tissue Journal of Virology. Jun, 2000 | Pubmed ID: 10799612 Preferential Coreceptor Utilization and Cytopathicity by Dual-tropic HIV-1 in Human Lymphoid Tissue Ex Vivo The Journal of Clinical Investigation. Sep, 1999 | Pubmed ID: 10487781 Ex Vivo Инфекции половых слизистой лимфоидной ткани человека и женщин с вирусом иммунодефицита человека 1 и Histoculture Andrea Introini1,2, Christophe Vanpouille2, Wendy Fitzgerald2, Kristina Broliden1, Leonid Margolis2 1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health JoVE 57013 Immunology and Infection Поток Virometry для анализа антигенные спектры вирионов и внеклеточной везикул Anush Arakelyan1, Wendy Fitzgerald1, Sonia Zicari1, Murad Vagida2, Jean-Charles Grivel3, Leonid Margolis1 1Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center Immunology and Infection
Ex Vivo Инфекции половых слизистой лимфоидной ткани человека и женщин с вирусом иммунодефицита человека 1 и Histoculture Andrea Introini1,2, Christophe Vanpouille2, Wendy Fitzgerald2, Kristina Broliden1, Leonid Margolis2 1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health JoVE 57013 Immunology and Infection
Поток Virometry для анализа антигенные спектры вирионов и внеклеточной везикул Anush Arakelyan1, Wendy Fitzgerald1, Sonia Zicari1, Murad Vagida2, Jean-Charles Grivel3, Leonid Margolis1 1Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center Immunology and Infection
