Liangliang Sun Department of Chemistry Michigan State University Biography Publications Institution JoVE Articles Liangliang Sun Dr. Liangliang Sun is an Assistant Professor in the Department of Chemistry at Michigan State University (East Lansing, Michigan, USA). He received his Ph.D. in Analytical Chemistry from Dalian Institute of Chemical Physics, Chinese Academy of Sciences (Dalian, China) in 2011. Then he worked with Prof. Norman Dovichi as a postdoctoral fellow and later as a research assistant professor from 2011 to 2016 at the University of Notre Dame (Notre Dame, Indiana, USA). He joined Michigan State University as an Assistant Professor in 2016. He has 12-years’ experiences on proteomics and has published 84 peer-reviewed papers with over 1700 citations.Dr. Sun’s research group at MSU aims to develop novel analytical methodologies based on capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) for comprehensive and highly sensitive bottom-up and top-down proteomics. His research group applies those methodologies for quantitative proteomics of zebrafish embryos and blastomeres during early embryogenesis to achieve the dynamics of gene expression and protein post-translational modifications (PTMs) during early embryogenesis at high time resolution and single-cell resolution. Publications Microscale Reversed-Phase Liquid Chromatography/Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Deep and Highly Sensitive Bottom-Up Proteomics: Identification of 7500 Proteins with Five Micrograms of an MCF7 Proteome Digest Analytical Chemistry. Sep, 2018 | Pubmed ID: 30102516 Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography-Capillary Zone Electrophoresis-Tandem Mass Spectrometry Analytical Chemistry. Sep, 2018 | Pubmed ID: 30085653 Asymmetric Distribution of Biomolecules of Maternal Origin in the Xenopus Laevis Egg and Their Impact on the Developmental Plan Scientific Reports. May, 2018 | Pubmed ID: 29844480 Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia Coli Proteome Analytical Chemistry. May, 2018 | Pubmed ID: 29620868 Systematic Evaluation of Immobilized Trypsin-Based Fast Protein Digestion for Deep and High-Throughput Bottom-Up Proteomics Proteomics. May, 2018 | Pubmed ID: 29577644 Strong Cation Exchange-reversed Phase Liquid Chromatography-capillary Zone Electrophoresis-tandem Mass Spectrometry Platform with High Peak Capacity for Deep Bottom-up Proteomics Analytica Chimica Acta. Jul, 2018 | Pubmed ID: 29475469 Quantitative Proteomics After Spinal Cord Injury (SCI) in a Regenerative and a Nonregenerative Stage in the Frog Molecular & Cellular Proteomics : MCP. Apr, 2018 | Pubmed ID: 29358338 Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry Analytical Chemistry. Jan, 2018 | Pubmed ID: 29231704 Phosphorylation Dynamics Dominate the Regulated Proteome During Early Xenopus Development Scientific Reports. Nov, 2017 | Pubmed ID: 29142207 Single-Shot Top-Down Proteomics with Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry for Identification of Nearly 600 Escherichia Coli Proteoforms Analytical Chemistry. Nov, 2017 | Pubmed ID: 29064224 Capillary Zone Electrophoresis-mass Spectrometry with Microliter-scale Loading Capacity, 140 Min Separation Window and High Peak Capacity for Bottom-up Proteomics The Analyst. Jun, 2017 | Pubmed ID: 28513658 Predicting Electrophoretic Mobility of Tryptic Peptides for High-Throughput CZE-MS Analysis Analytical Chemistry. Feb, 2017 | Pubmed ID: 28208305 Evaluation of a Commercial Electro-kinetically Pumped Sheath-flow Nanospray Interface Coupled to an Automated Capillary Zone Electrophoresis System Analytical and Bioanalytical Chemistry. Mar, 2017 | Pubmed ID: 27981343 Simplified Capillary Isoelectric Focusing with Chemical Mobilization for Intact Protein Analysis Journal of Separation Science. Feb, 2017 | Pubmed ID: 27935257 Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry Elijah N. McCool*1, Rachele Lubeckyj*1, Xiaojing Shen1, Qiang Kou2, Xiaowen Liu2,3, Liangliang Sun1 1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine JoVE 58644 Chemistry
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry Elijah N. McCool*1, Rachele Lubeckyj*1, Xiaojing Shen1, Qiang Kou2, Xiaowen Liu2,3, Liangliang Sun1 1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine JoVE 58644 Chemistry
