Other Publications (1)
Articles by Linn Sgier in JoVE
Characterization of Aquatic Biofilms with Flow Cytometry Linn Sgier1, Stephanie N. Merbt1, Ahmed Tlili1, Alexandra Kroll1, Anze Zupanic1 1Department of Environmental Toxicology, Eawag - Swiss Federal Institute for Aquatic Science and Technology Flow cytometry in combination with visual clustering offers an easy-to-use and fast method for studying aquatic biofilms. It can be used for biofilm characterization, detection of changes in biofilm community structure, and detection of abiotic particles embedded in the biofilm.
Other articles by Linn Sgier on PubMed
Flow Cytometry Combined with ViSNE for the Analysis of Microbial Biofilms and Detection of Microplastics Nature Communications. | Pubmed ID: 27188265 Biofilms serve essential ecosystem functions and are used in different technical applications. Studies from stream ecology and waste-water treatment have shown that biofilm functionality depends to a great extent on community structure. Here we present a fast and easy-to-use method for individual cell-based analysis of stream biofilms, based on stain-free flow cytometry and visualization of the high-dimensional data by viSNE. The method allows the combined assessment of community structure, decay of phototrophic organisms and presence of abiotic particles. In laboratory experiments, it allows quantification of cellular decay and detection of survival of larger cells after temperature stress, while in the field it enables detection of community structure changes that correlate with known environmental drivers (flow conditions, dissolved organic carbon, calcium) and detection of microplastic contamination. The method can potentially be applied to other biofilm types, for example, for inferring community structure for environmental and industrial research and monitoring.