In JoVE (1)
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Articles by Lucas Gonzalez-Nieto in JoVE
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining Lucas Gonzalez-Nieto1, Aline Domingues1, Michael Ricciardi1, Martin J. Gutman1, Helen S. Maxwell1, Nuria Pedreño-Lopez1, Varian Bailey1, Diogo M. Magnani1, Mauricio A. Martins1 1Department of Pathology, University of Miami Here, we present an optimized protocol for enumerating and characterizing rhesus macaque CD8+ T cells against the AIDS virus. This article is useful not only to the field of HIV immunology, but also to other areas of biomedical research where CD8+ T cell responses are known to affect disease outcome.