Luis Netto Departament of Genetics and Evolutionary Biology University of Sao Paulo Biography Publications Institution JoVE Articles Luis Netto I attended the University of Sao Paulo (Bacharel Degree in Biology, 1987; Ph.D. in 1992, under the guidance of Dr. Ohara Augusto) and performed an interchanged program in the Eppley Institute - Nebraska, in collaboration with Dr. Ercole Cavalieri. I was a NIH Postdoctoral Fellow, in collaboration with Dr. Stadtman from 1992 to 1996. I was Professor at State University of Campinas (Brazil) from 1996 to 1997. I am currently Full Professor at Biosciences Institute –University of Sao Paulo (Brazil). I received a prize from the Governor of Sao Paulo State for participation in the Xylella fastidiosa Genome Project (2000). I was head of Department of Genetics and Evolutionary Biology –2013-2017. I am member of the SfRBM council (since 2013).My research interests are on thiol-based proteins with emphasis in peroxiredoxin enzymes, using the yeast Saccharomyces cerevisiae as a model organism. The response to oxidative stress is also studied in bacteria, with emphasis in Xylella fastidiosa (bacterial plant pathogen) and Pseudomonas aeruginosa. We are characterizing biochemically and structurally a new class of antioxidant enzymes: Ohr (“Organic Hydroperoxide Resistance protein) that are potential targets for drug development. We pursue the structural and functional relationships of Cys-based proteins, employing multiple approaches. Publications Relevance of Peroxiredoxins in Pathogenic Microorganisms Applied Microbiology and Biotechnology. Jul, 2021 | Pubmed ID: 34258640 Oxidative Modification of Proteins: From Damage to Catalysis, Signaling, and Beyond Antioxidants & Redox Signaling. Apr, 2021 | Pubmed ID: 33726509 Molecular Structure and Functional Analysis of Pyocin S8 from Pseudomonas Aeruginosa Reveals the Essential Requirement of a Glutamate Residue in the H-N-H Motif for DNase Activity Journal of Bacteriology. 10, 2020 | Pubmed ID: 32817098 Reduction of Sulfenic Acids by Ascorbate in Proteins, Connecting Thiol-dependent to Alternative Redox Pathways Free Radical Biology & Medicine. 08, 2020 | Pubmed ID: 32615144 The Peroxidatic Thiol of Peroxiredoxin 1 is Nitrosated by Nitrosoglutathione but Coordinates to the Dinitrosyl Iron Complex of Glutathione Antioxidants (Basel, Switzerland). Mar, 2020 | Pubmed ID: 32218363 The Bicarbonate/carbon Dioxide Pair Increases Hydrogen Peroxide-mediated Hyperoxidation of Human Peroxiredoxin 1 The Journal of Biological Chemistry. 09, 2019 | Pubmed ID: 31366734 Mutations of Cys and Ser Residues in the α5-subunit of the 20S Proteasome from Saccharomyces Cerevisiae Affects Gating and Chronological Lifespan Archives of Biochemistry and Biophysics. 05, 2019 | Pubmed ID: 30940569 Non-Mammalian Prdx6 Enzymes (Proteins with 1-Cys Prdx Mechanism) Display PLA₂ Activity Similar to the Human Orthologue Antioxidants (Basel, Switzerland). Mar, 2019 | Pubmed ID: 30832204 Rapid Peroxynitrite Reduction by Human Peroxiredoxin 3: Implications for the Fate of Oxidants in Mitochondria Free Radical Biology & Medicine. 01, 2019 | Pubmed ID: 30391677 Mild Exercise Differently Affects Proteostasis and Oxidative Stress on Motor Areas During Neurodegeneration: A Comparative Study of Three Treadmill Running Protocols Neurotoxicity Research. Feb, 2019 | Pubmed ID: 30276717 Global Analysis of Erythroid Cells Redox Status Reveals the Involvement of Prdx1 and Prdx2 in the Severity of Beta Thalassemia PloS One. Month, 2018 | Pubmed ID: 30521599 Analyses of the Three 1-Cys Peroxiredoxins from Aspergillus Fumigatus Reveal That Cytosolic Prx1 is Central to HO Metabolism and Virulence Scientific Reports. 08, 2018 | Pubmed ID: 30120327 Structural Insights on the Efficient Catalysis of Hydroperoxide Reduction by Ohr: Crystallographic and Molecular Dynamics Approaches PloS One. Month, 2018 | Pubmed ID: 29782551 Effects of Mild Running on Substantia Nigra During Early Neurodegeneration Journal of Sports Sciences. Jun, 2018 | Pubmed ID: 28895489 Proteolytic Cleavage by the Inner Membrane Peptidase (IMP) Complex or Oct1 Peptidase Controls the Localization of the Yeast Peroxiredoxin Prx1 to Distinct Mitochondrial Compartments The Journal of Biological Chemistry. 10, 2017 | Pubmed ID: 28821623 Functional and Evolutionary Characterization of Ohr Proteins in Eukaryotes Reveals Many Active Homologs Among Pathogenic Fungi Redox Biology. 08, 2017 | Pubmed ID: 28391181 Urate Hydroperoxide Oxidizes Human Peroxiredoxin 1 and Peroxiredoxin 2 The Journal of Biological Chemistry. 05, 2017 | Pubmed ID: 28348082 Ohr Plays a Central Role in Bacterial Responses Against Fatty Acid Hydroperoxides and Peroxynitrite Proceedings of the National Academy of Sciences of the United States of America. 01, 2017 | Pubmed ID: 28028230 Catalytic Thr or Ser Residue Modulates Structural Switches in 2-Cys Peroxiredoxin by Distinct Mechanisms Scientific Reports. 09, 2016 | Pubmed ID: 27629822 The Roles of Peroxiredoxin and Thioredoxin in Hydrogen Peroxide Sensing and in Signal Transduction Molecules and Cells. Jan, 2016 | Pubmed ID: 26813662 How PH Modulates the Dimer-decamer Interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily The Journal of Biological Chemistry. Mar, 2015 | Pubmed ID: 25666622 20S Proteasome Activity is Modified Via S-glutathionylation Based on Intracellular Redox Status of the Yeast Saccharomyces Cerevisiae: Implications for the Degradation of Oxidized Proteins Archives of Biochemistry and Biophysics. Sep, 2014 | Pubmed ID: 24813691 Redox Regulation of the Proteasome Via S-glutathionylation Redox Biology. Dec, 2013 | Pubmed ID: 24396728 The Essential Gene YMR134W from Saccharomyces Cerevisiae is Important for Appropriate Mitochondrial Iron Utilization and the Ergosterol Biosynthetic Pathway FEBS Letters. Sep, 2013 | Pubmed ID: 23892078 Analysis of the Organic Hydroperoxide Response of Chromobacterium Violaceum Reveals That OhrR is a Cys-based Redox Sensor Regulated by Thioredoxin PloS One. Month, 2012 | Pubmed ID: 23071722 Redox Control of 20S Proteasome Gating Antioxidants & Redox Signaling. Jun, 2012 | Pubmed ID: 22229461 The Promoter of Filamentation (POF1) Protein from Saccharomyces Cerevisiae is an ATPase Involved in the Protein Quality Control Process BMC Microbiology. Dec, 2011 | Pubmed ID: 22204397 Catalytic Properties of Thioredoxin Immobilized on Superparamagnetic Nanoparticles Journal of Inorganic Biochemistry. May, 2011 | Pubmed ID: 21470550 Ohr (organic Hydroperoxide Resistance Protein) Possesses a Previously Undescribed Activity, Lipoyl-dependent Peroxidase The Journal of Biological Chemistry. Jul, 2010 | Pubmed ID: 20463026 Superoxide Dismutase 1-mediated Production of Ethanol- and DNA-derived Radicals in Yeasts Challenged with Hydrogen Peroxide: Molecular Insights into the Genome Instability of Peroxiredoxin-null Strains The Journal of Biological Chemistry. Feb, 2009 | Pubmed ID: 19106092 Role of Glutaredoxin 2 and Cytosolic Thioredoxins in Cysteinyl-based Redox Modification of the 20S Proteasome The FEBS Journal. Jun, 2008 | Pubmed ID: 18435761 Reduction of 1-Cys Peroxiredoxins by Ascorbate Changes the Thiol-specific Antioxidant Paradigm, Revealing Another Function of Vitamin C Proceedings of the National Academy of Sciences of the United States of America. Mar, 2007 | Pubmed ID: 17360337 Dihydrolipoyl Dehydrogenase As a Source of Reactive Oxygen Species Inhibited by Caloric Restriction and Involved in Saccharomyces Cerevisiae Aging FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Jan, 2007 | Pubmed ID: 17110466 Reactive Cysteine in Proteins: Protein Folding, Antioxidant Defense, Redox Signaling and More Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP. Jul-Aug, 2007 | Pubmed ID: 17045551 Structural Insights into Enzyme-substrate Interaction and Characterization of Enzymatic Intermediates of Organic Hydroperoxide Resistance Protein from Xylella Fastidiosa Journal of Molecular Biology. Jun, 2006 | Pubmed ID: 16631787 Heat Stress Promotes Mitochondrial Instability and Oxidative Responses in Yeast Deficient in Thiazole Biosynthesis Research in Microbiology. Apr, 2006 | Pubmed ID: 16171982 Glutathione and Thioredoxin Peroxidases Mediate Susceptibility of Yeast Mitochondria to Ca(2+)-induced Damage Archives of Biochemistry and Biophysics. May, 2004 | Pubmed ID: 15081889 H(2)O(2) Generation in Saccharomyces Cerevisiae Respiratory Pet Mutants: Effect of Cytochrome C Free Radical Biology & Medicine. Jul, 2003 | Pubmed ID: 12853074 Thiol Enzymes Protecting Mitochondria Against Oxidative Damage Methods in Enzymology. Month, 2002 | Pubmed ID: 11885279 Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae Fernando Gomes1, Helena Turano1, Angélica Ramos1, Luis E. S. Netto1 1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo JoVE 62853 Biochemistry
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae Fernando Gomes1, Helena Turano1, Angélica Ramos1, Luis E. S. Netto1 1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo JoVE 62853 Biochemistry