Malgorzata Zakrzewska Department of Protein Engineering, Faculty of Biotechnology University of Wroclaw Biography Publications Institution JoVE Articles Malgorzata Zakrzewska has not added a biography. If you are Malgorzata Zakrzewska and would like to personalize this page please email our Author Liaison for assistance. Publications Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential International Journal of Molecular Sciences. Jun, 2020 | Pubmed ID: 32526859 FGFR1 Clustering with Engineered Tetravalent Antibody Improves the Efficiency and Modifies the Mechanism of Receptor Internalization Molecular Oncology. 09, 2020 | Pubmed ID: 32511887 Site-Specific, Stoichiometric-Controlled, PEGylated Conjugates of Fibroblast Growth Factor 2 (FGF2) with Hydrophilic Auristatin Y for Highly Selective Killing of Cancer Cells Overproducing Fibroblast Growth Factor Receptor 1 (FGFR1) Molecular Pharmaceutics. 07, 2020 | Pubmed ID: 32501706 Intracellular Partners of Fibroblast Growth Factors 1 and 2 - Implications for Functions Cytokine & Growth Factor Reviews. May, 2020 | Pubmed ID: 32475760 FHF1 is a Bona Fide Fibroblast Growth Factor That Activates Cellular Signaling in FGFR-dependent Manner Cell Communication and Signaling : CCS. May, 2020 | Pubmed ID: 32357892 Low Stability of Integrin-Binding Deficient Mutant of FGF1 Restricts Its Biological Activity Cells. 08, 2019 | Pubmed ID: 31443196 Differential Regulation of Fibroblast Growth Factor Receptor 1 Trafficking and Function by Extracellular Galectins Cell Communication and Signaling : CCS. 06, 2019 | Pubmed ID: 31208421 Cross-Talk Between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins Cells. 05, 2019 | Pubmed ID: 31091809 Crosstalk Between P38 and Erk 1/2 in Downregulation of FGF1-Induced Signaling International Journal of Molecular Sciences. Apr, 2019 | Pubmed ID: 31013829 Targeting Cellular Trafficking of Fibroblast Growth Factor Receptors As a Strategy for Selective Cancer Treatment Journal of Clinical Medicine. Dec, 2018 | Pubmed ID: 30577533 Specific Antibody Fragment Ligand Traps Blocking FGF1 Activity International Journal of Molecular Sciences. Aug, 2018 | Pubmed ID: 30134556 Translocation of Exogenous FGF1 and FGF2 Protects the Cell Against Apoptosis Independently of Receptor Activation Journal of Molecular Biology. 10, 2018 | Pubmed ID: 30099027 FGF2 Dual Warhead Conjugate with Monomethyl Auristatin E and α-Amanitin Displays a Cytotoxic Effect Towards Cancer Cells Overproducing FGF Receptor 1 International Journal of Molecular Sciences. Jul, 2018 | Pubmed ID: 30029518 Nanodiamonds As "artificial Proteins": Regulation of a Cell Signalling System Using Low Nanomolar Solutions of Inorganic Nanocrystals Biomaterials. 09, 2018 | Pubmed ID: 29879652 High Affinity Promotes Internalization of Engineered Antibodies Targeting FGFR1 International Journal of Molecular Sciences. May, 2018 | Pubmed ID: 29748524 Cytotoxic Conjugates of Fibroblast Growth Factor 2 (FGF2) with Monomethyl Auristatin E for Effective Killing of Cells Expressing FGF Receptors ACS Omega. Jul, 2017 | Pubmed ID: 30023704 Antibody-induced Dimerization of FGFR1 Promotes Receptor Endocytosis Independently of Its Kinase Activity Scientific Reports. 08, 2017 | Pubmed ID: 28769084 The Autoinhibitory Function of D1 Domain of FGFR1 Goes Beyond the Inhibition of Ligand Binding The International Journal of Biochemistry & Cell Biology. 08, 2017 | Pubmed ID: 28652212 High-Yield Site-Specific Conjugation of Fibroblast Growth Factor 1 with Monomethylauristatin E Via Cysteine Flanked by Basic Residues Bioconjugate Chemistry. 07, 2017 | Pubmed ID: 28598150 High-Affinity Internalizing Human ScFv-Fc Antibody for Targeting FGFR1-Overexpressing Lung Cancer Molecular Cancer Research : MCR. 08, 2017 | Pubmed ID: 28483948 Design and Characteristics of Cytotoxic Fibroblast Growth Factor 1 Conjugate for Fibroblast Growth Factor Receptor-targeted Cancer Therapy Drug Design, Development and Therapy. 2016 | Pubmed ID: 27563235 Identification of New FGF1 Binding Partners-Implications for Its Intracellular Function IUBMB Life. Mar, 2016 | Pubmed ID: 26840910 Efficient Production and Purification of Extracellular Domain of Human FGFR-Fc Fusion Proteins from Chinese Hamster Ovary Cells Protein Expression and Purification. Jul, 2014 | Pubmed ID: 24727156 Nucleolin Regulates Phosphorylation and Nuclear Export of Fibroblast Growth Factor 1 (FGF1) PloS One. 2014 | Pubmed ID: 24595027 Protease Resistant Variants of FGF1 with Prolonged Biological Activity Protein and Peptide Letters. May, 2014 | Pubmed ID: 24304385 FGF1-gold Nanoparticle Conjugates Targeting FGFR Efficiently Decrease Cell Viability Upon NIR Irradiation International Journal of Nanomedicine. 2012 | Pubmed ID: 23226697 Tailoring Small Proteins Towards Biomedical Applications Current Pharmaceutical Biotechnology. Nov, 2011 | Pubmed ID: 21902634 Clathrin- and Dynamin-independent Endocytosis of FGFR3--implications for Signalling PloS One. 2011 | Pubmed ID: 21779335 Longer Action Means Better Drug: Tuning Up Protein Therapeutics Biotechnology Advances. Jul-Aug, 2011 | Pubmed ID: 21443940 Increased Protein Stability of FGF1 Can Compensate for Its Reduced Affinity for Heparin The Journal of Biological Chemistry. Sep, 2009 | Pubmed ID: 19574212 Structure of a Highly Stable Mutant of Human Fibroblast Growth Factor 1 Acta Crystallographica. Section D, Biological Crystallography. Jan, 2009 | Pubmed ID: 19153468 Phosphorylation of Fibroblast Growth Factor (FGF) Receptor 1 at Ser777 by P38 Mitogen-activated Protein Kinase Regulates Translocation of Exogenous FGF1 to the Cytosol and Nucleus Molecular and Cellular Biology. Jun, 2008 | Pubmed ID: 18411303 FGF-1: from Biology Through Engineering to Potential Medical Applications Critical Reviews in Clinical Laboratory Sciences. 2008 | Pubmed ID: 18293181 Structural Requirements of FGF-1 for Receptor Binding and Translocation into Cells Biochemistry. Dec, 2006 | Pubmed ID: 17176056 Highly Stable Mutants of Human Fibroblast Growth Factor-1 Exhibit Prolonged Biological Action Journal of Molecular Biology. Sep, 2005 | Pubmed ID: 16126225 The Many Faces of Protease-protein Inhibitor Interaction The EMBO Journal. Apr, 2005 | Pubmed ID: 15775973 Design of Fully Active FGF-1 Variants with Increased Stability Protein Engineering, Design & Selection : PEDS. Aug, 2004 | Pubmed ID: 15469994 Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation Mateusz Adam Krzyscik1,2, Aleksandra Sokolowska-Wedzina1, Karolina Jendryczko1, Marta Pozniak1, Daria Nawrocka1, Natalia Porebska1, Malgorzata Zakrzewska1, Jacek Otlewski1, Anna Szlachcic1, Lukasz Opalinski1 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, 2Department of Protein Biotechnology, Faculty of Biotechnology, University of Wroclaw JoVE 61918 Biochemistry
Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation Mateusz Adam Krzyscik1,2, Aleksandra Sokolowska-Wedzina1, Karolina Jendryczko1, Marta Pozniak1, Daria Nawrocka1, Natalia Porebska1, Malgorzata Zakrzewska1, Jacek Otlewski1, Anna Szlachcic1, Lukasz Opalinski1 1Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, 2Department of Protein Biotechnology, Faculty of Biotechnology, University of Wroclaw JoVE 61918 Biochemistry
