In JoVE (1)
Articles by Malin Kele in JoVE
Integreringsfri avledning av humane inducerte pluripotente stamceller ved bruk av Laminin 521 Matrix Elias Uhlin1, Ana Marin Navarro1,2, Harriet Rönnholm1, Kelly Day1, Malin Kele1, Anna Falk1 1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Robust avledning av humant indusert pluripotent stamceller (hiPS) celler ble oppnådd ved å bruke ikke-integrering av Sendai virus (SeV) vektor-mediert omprogrammering av dermale fibroblaster. HiPS-cellevedlikehold og klonal ekspansjon ble utført ved hjelp av xenofri og kjemisk definerte kulturbetingelser med rekombinant human laminin 521 (LN-521) matriks og essensielt E8 (E8) medium.
Other articles by Malin Kele on PubMed
Generation of Human IPS Cell Line CTL07-II from Human Fibroblasts, Under Defined and Xeno-free Conditions Stem Cell Research. Nov, 2016 | Pubmed ID: 27789397 CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.
Derivation of Human IPS Cell Lines from Monozygotic Twins in Defined and Xeno Free Conditions Stem Cell Research. Jan, 2017 | Pubmed ID: 28395796 Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.