Articles by Manca Kovač Viršek in JoVE
Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis Manca Kovač Viršek1, Andreja Palatinus1, Špela Koren1, Monika Peterlin1, Petra Horvat2, Andrej Kržan2 1Sector for Marine Waters, Institute for water of the Republic of Slovenia, 2Laboratory for Polymer Chemistry and Technology, National Institute for Chemistry Slovenia The protocol below describes the methodology for: microplastics sampling on the sea surface, separation of microplastic and chemical identification of particles. This protocol is in line with the recommendations for microplastics monitoring published by the MSFD Technical Subgroup on Marine Litter.
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis Marilyne Pflieger1, Petra Makorič1, Manca Kovač Viršek2, Špela Koren2 1Laboratory for Environmental Research, University of Nova Gorica, 2Sector for Marine Waters, Institute for Water of the Republic of Slovenia Microplastics act as vector of potentially toxic organic contaminants with unpredictable effects. This protocol describes an alternative methodology for assessing the levels of organochlorine pesticides adsorbed on plastic pellets and identifying the polymer chemical structure. The focus is on pressurized fluid extraction and attenuated total reflectance Fourier transform infrared spectroscopy.
Other articles by Manca Kovač Viršek on PubMed
Mercury Induced Community Tolerance in Microbial Biofilms is Related to Pollution Gradients in a Long-term Polluted River Aquatic Toxicology (Amsterdam, Netherlands). Nov, 2013 | Pubmed ID: 24184840 The net toxicity of different forms of mercury, in the long-term during their transformation processes, leads to the selection of resistant bacterial cells and this result in community tolerance which is pollution induced. Accordingly, based on profiles of a bacterial community structure, analysis of Hg resistant culturable bacteria and quantification of merA genes, we assessed development of pollution induced community tolerance in a mercury-polluted gradient in the Idrijca River. TTGE analysis did not show effects of mercury pollution to bacterial community diversity, while quantification of merA genes showed that merA genes can be correlated precisely (R(2)=0.83) with the total concentration of mercury in the biofilm microbial communities in the pollution gradient.