In JoVE (2)
Other Publications (0)
Articles by Manoj Kumar Karuppusamy Rathinam in JoVE
In vitro Methylation Assay to Study Protein Arginine Methylation Rama Kamesh Bikkavilli1, Sreedevi Avasarala1, Michelle Van Scoyk1, Manoj Kumar Karuppusamy Rathinam1, Jordi Tauler1, Stanley Borowicz1,2, Robert A. Winn1,3 1Department of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, 2Department of Hematology and Oncology, University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center Protein arginine methylation, catalyzed by a class of enzymes viz., protein arginine methyl transferases (PRMTs), is the process of enzymatic addition of methyl group(s) to arginines within proteins. The in vitro methylation assay is the most dependable tool for assessing the methylation status of known or novel PRMT substrates.
The Soft Agar Colony Formation Assay Stanley Borowicz1, Michelle Van Scoyk2, Sreedevi Avasarala2, Manoj Kumar Karuppusamy Rathinam2, Jordi Tauler2, Rama Kamesh Bikkavilli2, Robert A. Winn2,3 1Department of Hematology and Oncology, University of Illinois at Chicago, 2Department of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center The soft agar colony formation assay is a method used to confirm cellular anchorage-independent growth in vitro. The goal of this protocol is to illustrate a stringent method for the detection of the tumorigenic potential of transformed cells and the tumor suppressive effects of proteins on transformed cells.