In JoVE (1)
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Articles by María Antonia Cid in JoVE
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos María José Blanco*1, Ana I.R. Learte*1, Miguel A. Marchena1, Emma Muñoz-Sáez1, María Antonia Cid1, Iván Rodríguez-Martín1, Cristina Sánchez-Camacho2,3 1Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research, Universidad Europea de Madrid Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.