In JoVE (1)
Other Publications (1)
Articles by Maria C. Piña-Barba in JoVE
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix Nayeli Rodríguez-Fuentes1, Olivia Reynoso-Ducoing2, Ana Rodríguez-Hernández2, Javier R. Ambrosio-Hernández2, Maria C. Piña-Barba1, Armando Zepeda-Rodríguez3, Marco A. Cerbón-Cervantes4, José Tapia-Ramírez5, Luz E. Alcantara-Quintana6 1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular, Cinvestav-IPN, 6Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.
Other articles by Maria C. Piña-Barba on PubMed
Nukbone® Promotes Proliferation and Osteoblastic Differentiation of Mesenchymal Stem Cells from Human Amniotic Membrane Biochemical and Biophysical Research Communications. May, 2013 | Pubmed ID: 23598057 Bovine bone matrix Nukbone® (NKB) is an osseous tissue-engineering biomaterial that retains its mineral and organic phases and its natural bone topography and has been used as a xenoimplant for bone regeneration in clinics. There are not studies regarding its influence of the NKB in the behavior of cells during the repairing processes. The aim of this research is to demonstrate that NKB has an osteoinductive effect in human mesenchymal stem cells from amniotic membrane (AM-hMSCs). Results indicated that NKB favors the AM-hMSCs adhesion and proliferation up to 7 days in culture as shown by the scanning electron microscopy and proliferation measures using an alamarBlue assay. Furthermore, as demonstrated by reverse transcriptase polymerase chain reaction, it was detected that two gene expression markers of osteoblastic differentiation: the core binding factor and osteocalcin were higher for AM-hMSCs co-cultured with NKB in comparison with cultivated cells in absence of the biomaterial. As the results indicate, NKB possess the capability for inducing successfully the osteoblastic differentiation of AM-hMSC, so that, NKB is an excellent xenoimplant option for repairing bone tissue defects.