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Articles by Maria Pia Testa in JoVE
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5 Maria Pia Testa1, Omar Alvarado1, Andrea Wournell1, Jonathan Lee2, Frederick T. Guilford3, Steven H. Henriksen2, Tom R. Phillips1,2 1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.