Articles by Martin O. Bohn in JoVE
High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.) Carrie Butts-Wilmsmeyer1, Nicole A. Yana1, Gurshagan Kandhola2, Kent D. Rausch2, Rita H. Mumm1, Martin O. Bohn1 1Department of Crop Sciences, University of Illinois at Urbana-Champaign, 2Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign Here, we present a microscale protocol for processing grain samples and for incorporating this microscale approach into a high-throughput analytical pipeline. This is a higher throughput adaptation of currently available protocols.
Other articles by Martin O. Bohn on PubMed
Genomic Prediction of Single Crosses in the Early Stages of a Maize Hybrid Breeding Pipeline G3 (Bethesda, Md.). | Pubmed ID: 27646704 Prediction of single-cross performance has been a major goal of plant breeders since the beginning of hybrid breeding. Recently, genomic prediction has shown to be a promising approach, but only limited studies have examined the accuracy of predicting single-cross performance. Moreover, no studies have examined the potential of predicting single crosses among random inbreds derived from a series of biparental families, which resembles the structure of germplasm comprising the initial stages of a hybrid maize breeding pipeline. The main objectives of this study were to evaluate the potential of genomic prediction for identifying superior single crosses early in the hybrid breeding pipeline and optimize its application. To accomplish these objectives, we designed and analyzed a novel population of single crosses representing the Iowa Stiff Stalk Synthetic/Non-Stiff Stalk heterotic pattern commonly used in the development of North American commercial maize hybrids. The performance of single crosses was predicted using parental combining ability and covariance among single crosses. Prediction accuracies were estimated using cross-validation and ranged from 0.28 to 0.77 for grain yield, 0.53 to 0.91 for plant height, and 0.49 to 0.94 for staygreen, depending on the number of tested parents of the single cross and genomic prediction method used. The genomic estimated general and specific combining abilities showed an advantage over genomic covariances among single crosses when one or both parents of the single cross were untested. Overall, our results suggest that genomic prediction of single crosses in the early stages of a hybrid breeding pipeline holds great potential to re-design hybrid breeding and increase its efficiency.
Concentration of Beneficial Phytochemicals in Harvested Grain of U.S. Yellow Dent Maize (Zea Mays L.) Germplasm Journal of Agricultural and Food Chemistry. | Pubmed ID: 28874047 Although previous studies have examined the concentration of various nutritional compounds in maize, little focus has been devoted to the study of commercial maize hybrids or their inbred parents. In this study, a genetically and phenotypically diverse set of maize hybrids and inbreds relevant to U.S. commercial maize germplasm was evaluated for its variability in phytochemical content. Total protein, unsaturated fatty acids, tocopherols, soluble phenolics, and insoluble-bound phenolics were evaluated in this study. Of these compounds, only soluble and insoluble-bound phenolic acids exhibited means and variances that were at least as large as the means and variances reported for other sets of germplasm. This suggests that selection for high phenolic acid content is possible in current U.S. commercial germplasm. In contrast, while the total protein, unsaturated fatty acid, or tocopherol content could possibly be improved using current U.S. commercial germplasm, the results of this study indicate that the incorporation of more diverse sources of germplasm would most likely result in quicker genetic gains.