In JoVE (2)
Other Publications (2)
Articles by Martina Sonego in JoVE
Nucleofection de roedores Neuroblastos para estudar neuroblasto Migração Katarzyna Falenta*1, Sangeetha Gajendra*2, Martina Sonego1, Patrick Doherty1, Giovanna Lalli1 1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London Migração neuroblastos é um passo crucial na neurogênese pós-natal. O protocolo aqui descrito pode ser utilizado para investigar o papel dos reguladores de candidatos de migração neuroblastos empregando DNA / RNA pequeno hairpin (shRNA) nucleofection e um ensaio de migração 3D com neuroblastos isolados do roedor pós-natal fluxo migratório rostral.
In vivo Eletroporação pós-natal e tempo-lapso de neuroblasto Migração na aguda fatias do cérebro do rato Martina Sonego*1, Ya Zhou*1, Madeleine Julie Oudin2, Patrick Doherty1, Giovanna Lalli1 1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology Migração neuroblastos é um evento fundamental para a neurogênese pós-natal. Nós descrevemos um protocolo para a rotulagem eficiente de neuroblastos por eletroporação in vivo pós-natal e visualização posterior de sua migração utilizando time-lapse de imagens de fatias cerebrais agudas. Nós incluímos uma descrição para a análise quantitativa da dinâmica neuroblastos por rastreamento de vídeo.
Other articles by Martina Sonego on PubMed
Fascin Regulates the Migration of Subventricular Zone-derived Neuroblasts in the Postnatal Brain The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jul, 2013 | Pubmed ID: 23884926 After birth, stem cells in the subventricular zone (SVZ) generate neuroblasts that migrate along the rostral migratory stream (RMS) to become interneurons in the olfactory bulb (OB). This migration is a fundamental event controlling the proper integration of new neurons in a pre-existing synaptic network. Many regulators of neuroblast migration have been identified; however, still very little is known about the intracellular molecular mechanisms controlling this process. Here, we show that the actin-bundling protein fascin is highly upregulated in mouse SVZ-derived migratory neuroblasts. Fascin-1ko mice display an abnormal RMS and a smaller OB. Bromodeoxyuridine labeling experiments show that lack of fascin significantly impairs neuroblast migration, but does not appear to affect cell proliferation. Moreover, fascin depletion substantially alters the polarized morphology of rat neuroblasts. Protein kinase C (PKC)-dependent phosphorylation of fascin on Ser39 regulates its actin-bundling activity. In vivo postnatal electroporation of phosphomimetic (S39D) or nonphosphorylatable (S39A) fascin variants followed by time-lapse imaging of brain slices demonstrates that the phospho-dependent modulation of fascin activity ensures efficient neuroblast migration. Finally, fluorescence lifetime imaging microscopy studies in rat neuroblasts reveal that the interaction between fascin and PKC can be modulated by cannabinoid signaling, which controls neuroblast migration in vivo. We conclude that fascin, whose upregulation appears to mark the transition to the migratory neuroblast stage, is a crucial regulator of neuroblast motility. We propose that a tightly regulated phospho/dephospho-fascin cycle modulated by extracellular signals is required for the polarized morphology and migration in neuroblasts, thus contributing to efficient neurogenesis.
Transcriptional Basis for the Inhibition of Neural Stem Cell Proliferation and Migration by the TGFβ-Family Member GDF11 PloS One. 2013 | Pubmed ID: 24244313 Signalling through EGF, FGF and endocannabinoid (eCB) receptors promotes adult neurogenesis, and this can be modelled in culture using the Cor-1 neural stem cell line. In the present study we show that Cor-1 cells express a TGFβ receptor complex composed of the ActRIIB/ALK5 subunits and that a natural ligand for this receptor complex, GDF11, activates the canonical Smad2/3 signalling cascade and significantly alters the expression of ∼4700 gene transcripts within a few hours of treatment. Many of the transcripts regulated by GDF11 are also regulated by the EGF, FGF and eCB receptors and by the MAPK pathway - however, in general in the opposite direction. This can be explained to some extent by the observation that GDF11 inhibits expression of, and signalling through, the EGF receptor. GDF11 regulates expression of numerous cell-cycle genes and suppresses Cor-1 cell proliferation; interestingly we found down-regulation of Cyclin D2 rather than p27kip1 to be a good molecular correlate of this. GDF11 also inhibited the expression of numerous genes linked to cytoskeletal regulation including Fascin and LIM and SH3 domain protein 1 (LASP1) and this was associated with an inhibition of Cor-1 cell migration in a scratch wound assay. These data demonstrate GDF11 to be a master regulator of neural stem cell transcription that can suppress cell proliferation and migration by regulating the expression of numerous genes involved in both these processes, and by suppressing transcriptional responses to factors that normally promote proliferation and/or migration.