In JoVE (1)
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Articles by Masaru Ikegami in JoVE
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation Keiji Itaka1, Satoshi Uchida1, Akitsugu Matsui1, Kayoko Yanagihara1, Masaru Ikegami1, Taisuke Endo2, Takehiko Ishii3, Kazunori Kataoka2 1Graduate School of Medicine, Laboratory of Clinical Biotechnology, The University of Tokyo, 2Graduate School of Engineering, Department of Materials Engineering, The University of Tokyo, 3Graduate School of Engineering, Department of Bioengineering, The University of Tokyo This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.