In JoVE (1)
Other Publications (1)
Articles by Matthew J. Wyatt in JoVE
التسليم المباشر للMorpholinos MIF في Otocyst اسماك الزرد عن طريق الحقن وElectroporation يؤثر على التنمية الداخلية الأذن Katie E. Holmes1, Matthew J. Wyatt2, Yu-chi Shen2, Deborah A. Thompson2,3, Kate F. Barald2,4 1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI وقد تم تطوير طريقة لإيصال morpholinos مباشرة في otocyst الزرد في 24hpf. microinjection به من morpholinos في تجويف الحويصلة الأذنية وelectroporation لإحداث الاختراق ، وكنا قادرين على تجاوز تأثير morpholinos على الدماغ والحصول على آثار محددة على الأذن الداخلية.
Other articles by Matthew J. Wyatt on PubMed
Uniform Cell Seeding and Generation of Overlapping Gradient Profiles in a Multiplexed Microchamber Device with Normally-closed Valves Lab on a Chip. Nov, 2010 | Pubmed ID: 20835429 Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells.