Other Publications (1)
Articles by Melanie Uebelhoer in JoVE
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue David Jelinek1,2, Aimee Flores1,3, Melanie Uebelhoer1, Vincent Pasque2, Kathrin Plath2,3, M. Luisa Iruela-Arispe1,3, Heather R. Christofk2,3, William E. Lowry1,3, Hilary A. Coller1,2,3 1Department of Molecular, Cell and Developmental Biology, UCLA, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, UCLA We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.
Other articles by Melanie Uebelhoer on PubMed
Cross-talk Between Signaling and Metabolism in the Vasculature Vascular Pharmacology. Aug, 2016 | Pubmed ID: 27291139 The link between signaling and metabolism was first recognized with insulin signal transduction. Efficient glucose uptake by the endothelium requires insulin receptor activation to deliver GLUT receptors to the cell surface. More recently however, additional evidence has emerged for a broader crosstalk as signaling events have been shown to regulate a large number of metabolic enzymes. Changes in the metabolic status of endothelial and smooth muscle cells are observed at times of increased proliferative activity and these coincide with activation of cell surface receptors. Intriguingly, a rise in glycolysis appears to be associated with remodeling of the actin cytoskeleton during migration and angiogenesis. Overall, understanding how do signaling and metabolic pathways intersect and cross-regulate each other has become an important question and an emerging cornerstone in vascular biology.