Articles by Melissa J. Walker in JoVE
Gecontroleerde Cervicale Laceration Letsel in Muizen Yi Ping Zhang1, Melissa J. Walker2, Lisa B. E. Shields1, Xiaofei Wang2, Chandler L. Walker2, Xiao-Ming Xu2, Christopher B. Shields1 1Norton Neuroscience Institute, Norton Healthcare, 2Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Medical Neuroscience Graduate Program, and Department of Anatomy and Cell Biology, Indiana University School of Medicine Een nieuwe techniek om een reproduceerbare creëren
A Novel Vertebral Stabilization werkwijze voor het produceren Contusive Spinal Cord Injury Melissa J. Walker*1,2, Chandler L. Walker*1,3, Y. Ping Zhang4, Lisa B. E. Shields4, Christopher B. Shields4, Xiao-Ming Xu1,3 1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Indiana University School of Medicine, 2Medical Neuroscience Graduate Program, Indiana University School of Medicine, 3Department of Anatomy and Cell Biology, Indiana University School of Medicine, 4Norton Neuroscience Institute, Norton Healthcare Vertebrale stabilisatie is noodzakelijk voor het minimaliseren van variabiliteit en voor het produceren van consistente experimentele ruggenmergletsels. Met een aangepaste stabiliserende inrichting in combinatie met de NYU / Mascis slaginrichting, hebben we hier aangetoond dat de juiste apparatuur en procedure voor het genereren reproduceerbare hemi-contusive cervicale (C5) ruggenmergletsel bij volwassen ratten.
Other articles by Melissa J. Walker on PubMed
FMRI of the Brain's Response to Stimuli Experimentally Paired with Alcohol Intoxication Psychopharmacology. Apr, 2012 | Pubmed ID: 21993878 Individuals learn associations between alcohol's sensory properties and intoxication, with such conditioned stimuli (CS) becoming involved in craving and relapse. However, these CS also carry idiosyncratic associations.
Systemic Bisperoxovanadium Activates Akt/mTOR, Reduces Autophagy, and Enhances Recovery Following Cervical Spinal Cord Injury PloS One. 2012 | Pubmed ID: 22253859 Secondary damage following primary spinal cord injury extends pathology beyond the site of initial trauma, and effective management is imperative for maximizing anatomical and functional recovery. Bisperoxovanadium compounds have proven neuroprotective effects in several central nervous system injury/disease models, however, no mechanism has been linked to such neuroprotection from bisperoxovanadium treatment following spinal trauma. The goal of this study was to assess acute bisperoxovanadium treatment effects on neuroprotection and functional recovery following cervical unilateral contusive spinal cord injury, and investigate a potential mechanism of the compound's action. Two experimental groups of rats were established to 1) assess twice-daily 7 day treatment of the compound, potassium bisperoxo (picolinato) vanadium, on long-term recovery of skilled forelimb activity using a novel food manipulation test, and neuroprotection 6 weeks following injury and 2) elucidate an acute mechanistic link for the action of the drug post-injury. Immunofluorescence and Western blotting were performed to assess cellular signaling 1 day following SCI, and histochemistry and forelimb functional analysis were utilized to assess neuroprotection and recovery 6 weeks after injury. Bisperoxovanadium promoted significant neuroprotection through reduced motorneuron death, increased tissue sparing, and minimized cavity formation in rats. Enhanced forelimb functional ability during a treat-eating assessment was also observed. Additionally, bisperoxovanadium significantly enhanced downstream Akt and mammalian target of rapamycin signaling and reduced autophagic activity, suggesting inhibition of the phosphatase and tensin homologue deleted on chromosome ten as a potential mechanism of bisperoxovanadium action following traumatic spinal cord injury. Overall, this study demonstrates the efficacy of a clinically applicable pharmacological therapy for rapid initiation of neuroprotection post-spinal cord injury, and sheds light on the signaling involved in its action.